Reviewed, UniProtKB/Swiss-Prot Q6CMK4 (NMT_KLULA)

Names and origin

Protein names

Recommended name:
    Glycylpeptide N-tetradecanoyltransferase
    EC=2.3.1.97
Alternative name(s):
    Peptide N-myristoyltransferase
    Myristoyl-CoA:protein N-myristoyltransferase
      Short name=NMT

Gene names

Name:	NMT1
Ordered Locus Names:	KLLA0E19635g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Glycylpeptide N-tetradecanoyltransferase		PRO_0000064243
Regions
	Region	38 – 41	4	Myristoyl CoA-binding By similarity
	Region	168 – 204	37	Myristoyl CoA-binding By similarity
Sites
	Active site	447	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CMK4-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: B74AE4DC9A528D41	FASTA	447	52,323
	10 20 30 40 50 60 MSEEDKSKKL EELLKLLKVH DGDLSKFTSQ QRKDMNEYKF WKTQPVTKFD EVIKKEGPID 70 80 90 100 110 120 SSKRPEDIPD TPLPLLGDFE WCTVDVNDEK QLEDVYVLLN ENYVEDKDST FRFNYSRDFL 130 140 150 160 170 180 NWGLKPPGWK EEWQVGVRVK ETKRLVGFIS AIPTNLQVRG NDVRSVEINF LCVHKKLRSK 190 200 210 220 230 240 RLAPILIKEV TRRVNKYDIW QALHTGGVVL PSPVSSCRYA HRPLNWSKLY DVEFTALPAN 250 260 270 280 290 300 ATKTQMIAKY TLPKTPISNI KLMEERHVDE AFELFNKYQQ RFELRPNFDK EEFRHWILTR 310 320 330 340 350 360 EDVVYSYIIE NDEGKVTDFV SFYSLPFTII NNPLYKDLGI GYMFYYASDA DFGYDRFSAE 370 380 390 400 410 420 GTERLRKRLN LLINDACILA RNLKMDVFNA LTSQDNALFL EDLKFGPGDG FLNFYLFNYR 430 440 CFPITGGIKE DQTFDVEKRS NVGVVLL

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382125 Genomic DNA. Translation: CAG99922.1.
RefSeq	XP_454835.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CMK4.
Genome annotation databases
GeneID	2894734.
GenomeReviews	Gene locus KLLA0E19635g in contig CR382125_GR.
KEGG	kla:KLLA0E19635g.
Phylogenomic databases
HOGENOM	Q6CMK4.
OMA	EDDEAMF.
Enzyme and pathway databases
BRENDA	2.3.1.97. 74088.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. Myristoyl_trans. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT_KLULA

Accession

Primary (citable) accession number: Q6CMK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)