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Q6CLS2

- LIPA_KLULA

UniProt

Q6CLS2 - LIPA_KLULA

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Protein

Lipoyl synthase, mitochondrial

Gene
KLLA0F00836g
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi109 – 1091Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi115 – 1151Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi135 – 1351Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi139 – 1391Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi142 – 1421Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Ordered Locus Names:KLLA0F00836g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome F

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 370Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398268
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Interactioni

Protein-protein interaction databases

STRINGi28985.Q6CLS2.

Structurei

3D structure databases

ProteinModelPortaliQ6CLS2.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG79KPR7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6CLS2-1 [UniParc]FASTAAdd to Basket

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MLKPGIRSVQ KRFITETVVR STQPSVSRRR KTTHFTDKLN KGPSFEDFVN    50
GNAQKFTLDP LEKARQNSEE VQRLPTWLKV PIAKGSNFHK LKNDVKELKL 100
STVCEEAKCP NIGECWGGGD KSKATATIML LGDTCTRGCR FCSVKTNRKP 150
SAPDPTEPEN TAEAISRWGL GYVVLTTVDR DDLVDGGAYH LAETVQKIKQ 200
KAPNILVETL SGDFRGDLEM VKVMALSGLD VYAHNMETVE ALTPHVRDRR 250
ATYRQSLSVL ECAKKTVPTL VTKTSVMLGL GETDEQVLQT MKDLRAIGCD 300
VITFGQYMRP TKRHMKVVEY VTPEKFDYWK QKALELGFLY CASGPLVRSS 350
YKAGESFIEN VLRGRSRARI 370
Length:370
Mass (Da):41,470
Last modified:August 16, 2004 - v1
Checksum:i86A501949025496B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382126 Genomic DNA. Translation: CAG97824.1.
RefSeqiXP_455117.1. XM_455117.1.

Genome annotation databases

GeneIDi2895837.
KEGGikla:KLLA0F00836g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382126 Genomic DNA. Translation: CAG97824.1 .
RefSeqi XP_455117.1. XM_455117.1.

3D structure databases

ProteinModelPortali Q6CLS2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 28985.Q6CLS2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2895837.
KEGGi kla:KLLA0F00836g.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG79KPR7.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Entry informationi

Entry nameiLIPA_KLULA
AccessioniPrimary (citable) accession number: Q6CLS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: August 16, 2004
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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