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Protein

Pre-mRNA-processing ATP-dependent RNA helicase PRP5

Gene

PRP5

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent RNA helicase involved spliceosome assembly and in nuclear splicing. Catalyzes an ATP-dependent conformational change of U2 snRNP. Bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi290 – 2978ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing ATP-dependent RNA helicase PRP5 (EC:3.6.4.13)
Gene namesi
Name:PRP5
Ordered Locus Names:KLLA0F10505g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome F

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 812812Pre-mRNA-processing ATP-dependent RNA helicase PRP5PRO_0000232365Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_455558.1.

Structurei

3D structure databases

ProteinModelPortaliQ6CKI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini277 – 456180Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini481 – 639159Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi245 – 27430Q motifAdd
BLAST
Motifi404 – 4074DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0334. Eukaryota.
ENOG410XSQV. LUCA.
HOGENOMiHOG000007229.
InParanoidiQ6CKI1.
KOiK12811.
OMAiQCVLFSA.
OrthoDBiEOG092C1B4A.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6CKI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQTEKLRIR QEKLAKWKKQ KLNNDVTASV SVDAKEERLK KLEAWKKKKK
60 70 80 90 100
QQDELKQKDI KLTSIKSADR DRNKRRGQCS RKRPAFGGSD SESDDENSTS
110 120 130 140 150
TLFKPRRNDT NTHVLTKDNS KDDLDDGNDA LDEYIHKLID KDSNTNRVRL
160 170 180 190 200
IDNISEDEDA NDSSSEKTIS QEEAPVDSEI LFKKKGRKKI KAIDYSKVLN
210 220 230 240 250
LITLNKCLYR EPNDLGLMSE KDVEELRLSL DNIKISGKDC PKPVTKWSQL
260 270 280 290 300
GLSSEIMDLI SNELQFVTLT PIQCQAIPAI MSGRDVIGIS KTGSGKTVAF
310 320 330 340 350
LLPLVRQIKA QPPLAPDETG PIGLILTPTR ELAVQIQEEA LKFCKGSGIS
360 370 380 390 400
SICCVGGSEL KQQINELKRG VDIIVATPGR FIDLMTLNSG HLLSPTRISF
410 420 430 440 450
VVMDEADRLF DLGFGPQVNQ IMGCIRPDKQ CVLFSATFPS KLKHFASRTL
460 470 480 490 500
KNPIQITINS KSLINENIQQ RVQIFDEEHV KFEFLLKRLS DRLALHRGED
510 520 530 540 550
EKTIIFVGSQ QLCDLLYDEL LLNGITTFPI HAGKPSAERL RNLQKFKETD
560 570 580 590 600
NGILICTEVL SRGLNVPEVS LVIIYNAAKT IAQYVHTVGR TGRGTNNGVA
610 620 630 640 650
LSFVMVDELA SAYILVKCMK ENELSSVPMV VYQKLKEMND EFSSGLKTGK
660 670 680 690 700
YRLIQGFGGK GLDHLDELNE AKQSQEYTDY GVVENEEDVT EEESGATKME
710 720 730 740 750
YTRGKREEGH STTYFAHINI NDLPQLARWE ATKTETVSNI KQETGCNFES
760 770 780 790 800
KGSFYPEGKG PKNDTDDPKL YLIVEGAEES DVTMALELLN TKVKEGIRKA
810
AVKDIQSGKY RM
Length:812
Mass (Da):91,154
Last modified:August 16, 2004 - v1
Checksum:i0AF3B4F17D755C3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382126 Genomic DNA. Translation: CAG98266.1.
RefSeqiXP_455558.1. XM_455558.1.

Genome annotation databases

EnsemblFungiiCAG98266; CAG98266; KLLA0_F10505g.
GeneIDi2895725.
KEGGikla:KLLA0F10505g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382126 Genomic DNA. Translation: CAG98266.1.
RefSeqiXP_455558.1. XM_455558.1.

3D structure databases

ProteinModelPortaliQ6CKI1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_455558.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG98266; CAG98266; KLLA0_F10505g.
GeneIDi2895725.
KEGGikla:KLLA0F10505g.

Phylogenomic databases

eggNOGiKOG0334. Eukaryota.
ENOG410XSQV. LUCA.
HOGENOMiHOG000007229.
InParanoidiQ6CKI1.
KOiK12811.
OMAiQCVLFSA.
OrthoDBiEOG092C1B4A.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP5_KLULA
AccessioniPrimary (citable) accession number: Q6CKI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: August 16, 2004
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.