Reviewed, UniProtKB/Swiss-Prot Q6CKI0 (MCES_KLULA)

Names and origin

Protein names

Recommended name:
    mRNA cap guanine-N7 methyltransferase
    EC=2.1.1.56
Alternative name(s):
    mRNA (guanine-N(7)-)-methyltransferase
    mRNA cap methyltransferase

Gene names

Name:	ABD1
Ordered Locus Names:	KLLA0F10527g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for methylating the 5'-cap structure of mRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	mRNA cap guanine-N7 methyltransferase		PRO_0000303909

Sequences

	Sequence		Length	Mass (Da)
	Q6CKI0-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 9F207A1D9B7D2A57	FASTA	426	49,718
	10 20 30 40 50 60 MVLLPEKPVW MSQQQYEEQY GSLLKLKESE KCNHEDKQLA LQPKESSIGY STQERIAKLS 70 80 90 100 110 120 VPEVQDDDKN SKVRNRKHQR FDLEEKKKKL RLQKTIEEQI KHHDIEMTAN RVVNVDHVVR 130 140 150 160 170 180 QHYNERTFLS KKHNRNYSPI IKLRNFNNAI KYILIDKFTR AGDVVLELAC GKGGDLRKYG 190 200 210 220 230 240 AAGISQFIGI DISNASITEA LKRYHSMKNL EYQVILITGD CFGESLGVAV ESFPECRFPC 250 260 270 280 290 300 DIVSCQFALH YAFETEEKAR RMLLNVVKSL KIGGYFFGTI PDSEFIRYKM NKIPESVEKP 310 320 330 340 350 360 SWGNSIYKVT FSNNEYQKNG NEFPSPFGQM YTFWLEDAID NVPEYVIPFE SFRSLADEYG 370 380 390 400 410 420 MELELQKGFN EFFVEEIPNW VNRFSPKMRE GLKRSDGRYG VEGVEKEPAA YFYTTFAFRK VRDYQE

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382126 Genomic DNA. Translation: CAG98267.1.
RefSeq	XP_455559.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2895726.
KEGG	kla:KLLA0F10527g.
Phylogenomic databases
HOGENOM	Q6CKI0.
OMA	Q6CKI0. WLEDAID.
Enzyme and pathway databases
BRENDA	2.1.1.56. 74088.
Family and domain databases
InterPro	IPR016899. mRNA_G-N7_MeTrfase. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
PIRSF	PIRSF028762. ABD1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCES_KLULA

Accession

Primary (citable) accession number: Q6CKI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)