Q6CJC2 (ERG27_KLULA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-keto-steroid reductase
EC=1.1.1.270

Gene names

Name:	ERG27
Ordered Locus Names:	KLLA0F19756g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols By similarity.
Catalytic activity	4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP+ = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-keto sterol reductase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	3-keto-steroid reductase		PRO_0000054593
Sites
	Active site	201	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CJC2 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 7986B74A9B8920F1	FASTA	346	39,395
	10 20 30 40 50 60 MSSSKVAVVT GTNSNLGLNI VYRLIERSEP EDNLTIVVTS RTLPRVRECI DLIKAFVNTI 70 80 90 100 110 120 NRTGSVDYDY LLVDFTNMIS ILDAHYSLSK RYDHINYFFV NAAQGVYSGI DWLGAVKEVF 130 140 150 160 170 180 SSPLEAVTNP TYKIQRVGVK SKDGMGLVFQ ANVFGPYYLI QKLLPLLQAG QGTVVWVSSI 190 200 210 220 230 240 MSAPKYLSLQ DIQLLESDVS YEGSKRLVDL LHSATYKEMK KLGIRQYLTH PGIFTSLSFF 250 260 270 280 290 300 QYLNVFTYYG MLFLFYLARW IGSPWHNIQG YKAANAPVYV ATMANPHFEK EQMKYGSATF 310 320 330 340 RDGLEYIKTD EVDTTGCEDV YKYISNLKLQ WDEKLKDQIK PTRIPL

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382126 Genomic DNA. Translation: CAG98675.1.
RefSeq	XP_455967.1. XM_455967.1.
3D structure databases
ProteinModelPortal	Q6CJC2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CJC2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2895502.
GenomeReviews	Gene locus KLLA0F19756g in contig CR382126_GR.
KEGG	kla:KLLA0F19756g.
Phylogenomic databases
eggNOG	fuNOG05767.
HOGENOM	HBG398311.
OMA	FLFYLAR.
OrthoDB	EOG4FBN2N.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K09827.
ProtoNet	Search...

Entry information

Entry name

ERG27_KLULA

Accession

Primary (citable) accession number: Q6CJC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	August 16, 2004
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program