Reviewed, UniProtKB/Swiss-Prot Q6CJA8 (HOG1_KLULA)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase HOG1
      Short name=MAP kinase HOG1
    EC=2.7.11.24

Gene names

Name:	HOG1
Ordered Locus Names:	KLLA0F20053g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-173 and Tyr-175, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Mitogen-activated protein kinase HOG1		PRO_0000289694
Regions
	Domain	22 – 301	280	Protein kinase
	Nucleotide binding	28 – 36	9	ATP By similarity
	Motif	173 – 175	3	TXY
	Compositional bias	374 – 403	30	Gln-rich
Sites
	Active site	143	1	Proton acceptor By similarity
	Binding site	51	1	ATP By similarity
Amino acid modifications
	Modified residue	173	1	Phosphothreonine By similarity
	Modified residue	175	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CJA8-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: CEA1CB7A1849891D	FASTA	444	50,298
	10 20 30 40 50 60 MSNEEFIRTQ IFGTVFEITN RYTNLNPVGM GAFGLVCSAT DTLTSQPVAI KKIMKPFSTS 70 80 90 100 110 120 VLAKRTYREL KLLKHLRHEN LICLEDIFLS PLEDIYFVTE LQGTDLHRLL QTRPLEKQFV 130 140 150 160 170 180 QYFLYQILRG LKYVHSAGVI HRDLKPSNIL INENCDLKIC DFGLARIQDP QMTGYVSTRY 190 200 210 220 230 240 YRAPEIMLTW QKYNVEVDIW SAGCIFAEMI EGKPLFPGKD HVHQFSIITD LLGSPPKDVI 250 260 270 280 290 300 DTICSENTLK FVTSLPHRDP VPFSSRFQNL EPDAIDLLEK MLVFDPKKRI TAADALAHPY 310 320 330 340 350 360 LSPYHDPTDE PIAEAKFDWN FNDADLPVDT WRVMMYSEIL DFHQIGDPQI NTNATFDDQV 370 380 390 400 410 420 AAATVAAAEA ASKQQQQQQH QTEEQTQQTI ASTPPQAQVT PQQLESGANS NSNSNPSFSI 430 440 GPDPANETLT NFANQADQYV SKFK

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382126 Genomic DNA. Translation: CAG98689.1.
RefSeq	XP_455981.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2895492.
GenomeReviews	Gene locus KLLA0F20053g in contig CR382126_GR.
KEGG	kla:KLLA0F20053g.
Phylogenomic databases
HOGENOM	Q6CJA8.
OMA	SEILDFH.
Enzyme and pathway databases
BRENDA	2.7.11.24. 74088.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR008352. MAPK_p38. IPR000425. MIP. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01773. P38MAPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00221. MIP. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_KLULA

Accession

Primary (citable) accession number: Q6CJA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)