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Protein

4-aminobutyrate aminotransferase

Gene

KLLA0F20548g

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the degradation of gamma-aminobutyric acid (GABA), which is important for utilization of GABA as nitrogen source and for oxidative stress tolerance. Deaminates GABA to succinate semialdehyde, which in turn is converted to succinate by the succinate-semialdehyde dehydrogenase UGA2 (By similarity). May be involved in an alternative, arginase-independent arginine degradation pathway via GABA (Probable).By similarity1 Publication

Catalytic activityi

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.By similarity

Cofactori

pyridoxal 5'-phosphateBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei193 – 1931SubstrateBy similarity
Binding sitei352 – 3521Pyridoxal phosphate; shared with dimeric partnerBy similarity

GO - Molecular functioni

  1. 4-aminobutyrate transaminase activity Source: InterPro
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. gamma-aminobutyric acid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00073.

Names & Taxonomyi

Protein namesi
Recommended name:
4-aminobutyrate aminotransferaseBy similarity (EC:2.6.1.19By similarity)
Alternative name(s):
GABA aminotransferaseBy similarity
Short name:
GABA-ATBy similarity
Gamma-amino-N-butyrate transaminaseBy similarity
Short name:
GABA transaminase1 Publication
Gene namesi
Ordered Locus Names:KLLA0F20548g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)Imported
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome F

Subcellular locationi

Cytoplasm By similarity

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4724724-aminobutyrate aminotransferasePRO_0000432234Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei327 – 3271N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homodimer and homotetramer.By similarity

Protein-protein interaction databases

STRINGi28985.Q6CJ86.

Structurei

3D structure databases

ProteinModelPortaliQ6CJ86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1362Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000020208.
InParanoidiQ6CJ86.
KOiK13524.
OMAiTWCGDPA.
OrthoDBiEOG744TK4.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR004631. 4NH2But_aminotransferase_euk.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PTHR11986:SF6. PTHR11986:SF6. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6CJ86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAAKYYPN EPTEPKVVTS EIPGPESKAK VASLGEVFDS RPAYFVADYA
60 70 80 90 100
KSSGNYIVDV DGNKFLDVYA QISSIALGYN NPALIEAAKS DKMIRALVDR
110 120 130 140 150
PALGNFPGAD LEDILKQLLK FAPKGQNKIW SGLSGADANE LAFKAAFMYY
160 170 180 190 200
RQLQRGGHGI DFSEEENSSV MENTSPGSPQ LAVLSFKKAF HGRLFASGSS
210 220 230 240 250
TCSKPIHKLD FPAFNWPHGE YPVYKYPLSE NEEENKKEDD RCLAIVEDLI
260 270 280 290 300
KSWPTPVAAL IIEPIQSEGG DNHASKYFLQ SLRDLTSKYN VVYIIDEVQT
310 320 330 340 350
GVGATGKFWC HEWADIQPPV DLVTFSKKFQ SAGYWFHDDR FIPNKAYRQF
360 370 380 390 400
NTWCGDPARM IIAGAIGQEI VDNNLVDQCA RVGDYLFEKL EKLQAKYPTR
410 420 430 440 450
LINLRGKNRG TFIAFDLETS AERDQLLKLL KSNGCNVGGC AEKSVRLRPS
460 470
LTFEEKHADI FVDALEKSIG QL
Length:472
Mass (Da):52,575
Last modified:August 16, 2004 - v1
Checksum:iFB2A97AAFE334999
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382126 Genomic DNA. Translation: CAG98711.1.
RefSeqiXP_456003.1. XM_456003.1.

Genome annotation databases

GeneIDi2895485.
KEGGikla:KLLA0F20548g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382126 Genomic DNA. Translation: CAG98711.1.
RefSeqiXP_456003.1. XM_456003.1.

3D structure databases

ProteinModelPortaliQ6CJ86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi28985.Q6CJ86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2895485.
KEGGikla:KLLA0F20548g.

Phylogenomic databases

HOGENOMiHOG000020208.
InParanoidiQ6CJ86.
KOiK13524.
OMAiTWCGDPA.
OrthoDBiEOG744TK4.

Enzyme and pathway databases

UniPathwayiUPA00073.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR004631. 4NH2But_aminotransferase_euk.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PTHR11986:SF6. PTHR11986:SF6. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
  2. "An alternative, arginase-independent pathway for arginine metabolism in Kluyveromyces lactis involves guanidinobutyrase as a key enzyme."
    Romagnoli G., Verhoeven M.D., Mans R., Fleury Rey Y., Bel-Rhlid R., van den Broek M., Seifar R.M., Ten Pierick A., Thompson M., Muller V., Wahl S.A., Pronk J.T., Daran J.M.
    Mol. Microbiol. 93:369-389(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE FUNCTION, PATHWAY.

Entry informationi

Entry nameiGABAT_KLULA
AccessioniPrimary (citable) accession number: Q6CJ86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: August 16, 2004
Last modified: March 4, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Induced 4.7-fold in an arginase-negative strain during exponential growth.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.