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Protein

Enoyl-[acyl-carrier-protein] reductase, mitochondrial

Gene

ETR1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Required for respiration and the maintenance of the mitochondrial compartment.By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei77Proton donorBy similarity1
Binding sitei159NADPBy similarity1
Binding sitei375NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi187 – 190NADPBy similarity4
Nucleotide bindingi210 – 212NADPBy similarity3
Nucleotide bindingi285 – 288NADPBy similarity4
Nucleotide bindingi310 – 312NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNADP

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase, mitochondrial (EC:1.3.1.104)
Alternative name(s):
2-enoyl thioester reductase
Gene namesi
Name:ETR1
Ordered Locus Names:KLLA0F24552g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome F

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionSequence analysisAdd BLAST17
ChainiPRO_000000090218 – 382Enoyl-[acyl-carrier-protein] reductase, mitochondrialAdd BLAST365

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi284590.XP_456173.1.

Structurei

3D structure databases

ProteinModelPortaliQ6CIR6.
SMRiQ6CIR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0025. Eukaryota.
COG0604. LUCA.
HOGENOMiHOG000294683.
InParanoidiQ6CIR6.
KOiK07512.
OMAiAFRGFWM.
OrthoDBiEOG092C2NJA.

Family and domain databases

InterProiView protein in InterPro
IPR013149. ADH_C.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF00107. ADH_zinc_N. 1 hit.
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6CIR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFLSKRFL SFSQRAMSQE SLKFKSLIYS SHDSQDCTKV LKVHNYKPKK
60 70 80 90 100
GSETSIVLKT LAFPINPSDI NQLEGVYPSK PDKVTDYSTD EPSAIAGNEG
110 120 130 140 150
VFEVVSVPSS VSTLKPGDKV IPLQANFGTW STYRTCDKES DLIKIEGVDI
160 170 180 190 200
YTAATVAVNG CTAYQLVNDY INWDPKGNDW LVQNAGTSSV SKIVTQIAKA
210 220 230 240 250
KNINTLSVIR DRENFEEVAE ILEKKYGATK VISETENGEK EFGKEVLPKV
260 270 280 290 300
LGSNAQVKLA LNSVGGKSCA NIARKLSKDG LMLTYGGMSK QPLTFPTGLF
310 320 330 340 350
IFKGLKSHGF WVTENSKRDP ENKIKTVNEV IELYRDGKII SPKEDIRALE
360 370 380
WDVNNASDEE VLQLITDGIK TKGTKNMVIL KW
Length:382
Mass (Da):42,256
Last modified:August 16, 2004 - v1
Checksum:iDE17CC6087A6A8FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382126 Genomic DNA. Translation: CAG98881.1.
RefSeqiXP_456173.1. XM_456173.1.

Genome annotation databases

EnsemblFungiiCAG98881; CAG98881; KLLA0_F24552g.
GeneIDi2895202.
KEGGikla:KLLA0F24552g.

Similar proteinsi

Entry informationi

Entry nameiETR1_KLULA
AccessioniPrimary (citable) accession number: Q6CIR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: August 16, 2004
Last modified: September 27, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families