Reviewed, UniProtKB/Swiss-Prot Q6CIQ1 (CARA_KLULA)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase arginine-specific small chain
      Short name=CPS-A
    EC=6.3.5.5
Alternative name(s):
    Arginine-specific carbamoyl-phosphate synthetase, glutamine chain

Gene names

Name:	CPA1
Ordered Locus Names:	KLLA0F24882g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Carbamoyl-phosphate synthase arginine-specific small chain		PRO_0000290596
Regions
	Domain	197 – 389	193	Glutamine amidotransferase type-1
Sites
	Active site	277	1	Nucleophile By similarity
	Active site	362	1	By similarity
	Active site	364	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CIQ1-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: B08D9B8A9C973AD8	FASTA	409	44,921
	10 20 30 40 50 60 MPNFKSTAPK AKFAIYNNSN PDSESGFVEF QGYSFGAPVS CSGEAVFTTS LVGYPESMTD 70 80 90 100 110 120 PSYKGQILVF TQPLIGNYGV PSGEERDDFN LLKYLESPHI HVRGIVVAEY AWRYSHWTAV 130 140 150 160 170 180 ESLATWCQRE NVTAIGGIDT RAVVQILREQ GSSPSTITVQ GSFIPKPIKP ETDLVKIVST 190 200 210 220 230 240 KKPFFVKALS SVKTPFNVAL IDCGVKENII RCLVERGANV TVFPYDYPIT SVANQFDGIF 250 260 270 280 290 300 ISNGPGDPVQ CMESTVPELK KLLETNTLQD LPIFGICLGH QLLALASGGK TIKLGYGNRA 310 320 330 340 350 360 HNIPALDLTT GQCHITSQNH GYAVDVDTLP ESGFKPFFQN LNDKSNEGMI HVSRPIFSTQ 370 380 390 400 FHPEAKGGPR DSAVLFDRYF DNMSQYRALK GPKVKLTLNT SSLATERVF

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382126 Genomic DNA. Translation: CAG98896.1.
RefSeq	XP_456188.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CIQ1.
Genome annotation databases
GeneID	2895188.
GenomeReviews	Gene locus KLLA0F24882g in contig CR382126_GR.
KEGG	kla:KLLA0F24882g.
Phylogenomic databases
HOGENOM	Q6CIQ1.
OMA	LFDGSNC.
Enzyme and pathway databases
BRENDA	6.3.5.5. 74088.
Family and domain databases
InterPro	IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_KLULA

Accession

Primary (citable) accession number: Q6CIQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)