Reviewed, UniProtKB/Swiss-Prot Q6CIC9 (JHD1_KLULA)

Names and origin

Protein names

Recommended name:
    JmjC domain-containing histone demethylation protein 1
    EC=1.14.11.27
Alternative name(s):
    [Histone-H3]-lysine-36 demethylase 1

Gene names

Name:	JHD1
Ordered Locus Names:	KLLA0F27643g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code By similarity.
Catalytic activity	Protein N(6),N(6)-dimethyl-L-lysine + 2-oxoglutarate + O(2) = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO(2). Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O(2) = protein L-lysine + succinate + formaldehyde + CO(2).
Cofactor	Binds 1 Fe(2+) ion per subunit By similarity.
Subcellular location	NucleusBy similarity.
Domain	The JmjC domain mediates the demethylation activity By similarity.
Sequence similarities	Belongs to the JHDM1 histone demethylase family. Contains 1 JmjC domain. Contains 1 PHD-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Iron Metal-binding Zinc
Molecular function	Chromatin regulator Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	histone demethylase activity (H3-K36 specific) Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	JmjC domain-containing histone demethylation protein 1		PRO_0000226798
Regions
	Domain	242 – 401	160	JmjC
	Zinc finger	6 – 70	65	PHD-type
Sites
	Metal binding	297	1	Iron; catalytic By similarity
	Metal binding	299	1	Iron; catalytic By similarity
	Metal binding	369	1	Iron; catalytic By similarity
	Binding site	294	1	Substrate By similarity
	Binding site	314	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CIC9-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: F282BD749A1F1EF2	FASTA	478	54,963
	10 20 30 40 50 60 MTSGTVKCHF CKKDDSEDKG QPIWVGCEFC DGWCHLTCVP IQFVVPKLEN PSQLLAFKEK 70 80 90 100 110 120 HVESFKCTLH DESKCALLKL NGVSVGTEDI AFTKRNRLRN KRPIDYIALN EGNDKRLKHE 130 140 150 160 170 180 HPHTQAFLAC FEKWKDPKAI SSSELESDFQ IIKVPLRVSD PADSGMYVIS ANELGLVDSK 190 200 210 220 230 240 DHVKLNVEYL TKIMGDDYPL DVMDVQTQMN EKWTLSQWNE YYSHTSPSDR DRIRNVISLE 250 260 270 280 290 300 VSHVESFKDG IRRPNAVNNN DLVDIVWNFG RTETDIERPK VTKYILMSVG NAYTDFHLDF 310 320 330 340 350 360 AGTSVYYNVI SGSKKFILFP PTDYNLKKYR EWCDNDNQND IFLGDQLEAG IAMELTEGNL 370 380 390 400 410 420 FMIPCGYIHA VYTPEDSFIV GGNFLTLRDI TTQLNVVEIE HQTKVPKKFT FPQFESVMGK 430 440 450 460 470 TCEWLLNSDH IQSISSEDIE NLVKYLSSSN IKYKPINYQS KKELITELKN KIIKFEHT

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382126 Genomic DNA. Translation: CAG99018.1.
RefSeq	XP_456310.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2894869.
KEGG	kla:KLLA0F27643g.
Phylogenomic databases
HOGENOM	Q6CIC9.
Family and domain databases
InterPro	IPR003347. TF_JmjC_AAH. IPR001965. Znf_PHD. [Graphical view]
SMART	SM00558. JmjC. 1 hit. SM00249. PHD. 1 hit. [Graphical view]
PROSITE	PS51184. JMJC. 1 hit. PS01359. ZF_PHD_1. False negative. PS50016. ZF_PHD_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JHD1_KLULA

Accession

Primary (citable) accession number: Q6CIC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)