Reviewed, UniProtKB/Swiss-Prot Q6CHD6 (PMIP_YARLI)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
Ordered Locus Names:	YALI0A09988g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion Potential
	Chain	29 – 776	748	Mitochondrial intermediate peptidase		PRO_0000338597
Sites
	Active site	562	1	By similarity
	Metal binding	561	1	Zinc; catalytic By similarity
	Metal binding	565	1	Zinc; catalytic By similarity
	Metal binding	568	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CHD6-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 60F6B023BA9AEF67	FASTA	776	87,572
	10 20 30 40 50 60 MRRFSTLSRR LQRVVPASSA STANTSPSPA LYTGLEPKIK ESQDSLIRAV FDNGDVWQDF 70 80 90 100 110 120 SQKSVAKPKQ SRSITGFINY LTNESDYETG LFMNDFLKTP AGFQKYTAAS IEEAGQLIQQ 130 140 150 160 170 180 LLGALTQRDK LRHAITTFDR LSDVLCQVID LAEFIRAAHP EQHFVQAAQE AHEQMYEYMN 190 200 210 220 230 240 VLNTSVELYT VLDMVFKDSE IVNQLTHEEK VVGTLLLEDF KKSGVTLDDA GRENFVSLTT 250 260 270 280 290 300 KISLLGRDFI SSNHPKEDYI TLTQGEAQGL DPQLAQQLSQ GNSVYVPTGG VPGQLALRGM 310 320 330 340 350 360 KNENSRKLLW SKMRESSDKS IESLEDLLVS RLELANLMGK ESYADYLLSD KMAGNPENVM 370 380 390 400 410 420 RFLNGLLDKT LPGAKKELSV LEQIKKQATG NPKSILQAWD KSYYASQLLY QKRNKTKTAH 430 440 450 460 470 480 MLSEYFSVGT VVQGLSRIFD KIYGIRFVPT ETKTGETWHH DVRRLDVVSE TEGLIGIMYA 490 500 510 520 530 540 DLFQREGKSP NPAHFTVRCS REIYPDELAH MSSSPIAKVP TLNLNGKVFQ IPTIALICDF 550 560 570 580 590 600 TTPHDLYPSL LSYQEVETLF HEMGHAIHSM LGRTSLHNVC GTRCATDFVE LPSVFMENFA 610 620 630 640 650 660 SNPESLALFA RHYSSDSPLP YQQLERHLNE QSYFKDVEQY TQIKMAMLDQ VLHGNILKSI 670 680 690 700 710 720 TNGHFNSQKL YDNLEKDRPL FPPSPSSWHG SFGHLFGYGA SYYCYLLDRQ MADIVWKKLF 730 740 750 760 770 SKNPLSRDAG SRMKNEVLQW GGSRDPWECI AGVLEDPELA KGGSQAMEKI GNYDKH

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382127 Genomic DNA. Translation: CAG83853.1.
RefSeq	XP_499926.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CHD6.
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	2905768.
GenomeReviews	Gene locus YALI0A09988g in contig CR382127_GR.
KEGG	yli:YALI0A09988g.
Phylogenomic databases
HOGENOM	Q6CHD6.
OMA	AMGERYR.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_YARLI

Accession

Primary (citable) accession number: Q6CHD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)