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Protein

Deoxyhypusine synthase

Gene

DYS1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

NAD+By similarity

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231NADBy similarity
Binding sitei224 – 2241NADBy similarity
Binding sitei229 – 2291SpermidineBy similarity
Binding sitei271 – 2711NAD; via amide nitrogenBy similarity
Binding sitei276 – 2761SpermidineBy similarity
Active sitei317 – 3171NucleophileBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi91 – 955NADBy similarity
Nucleotide bindingi117 – 1193NADBy similarity
Nucleotide bindingi296 – 2972NADBy similarity
Nucleotide bindingi330 – 3312NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Short name:
DHS
Gene namesi
Name:DYS1
Ordered Locus Names:YALI0B00726g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
Proteomesi
  • UP000001300 Componenti: Chromosome B

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Deoxyhypusine synthasePRO_0000134487Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6CG56.
SMRiQ6CG56. Positions 5-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 1232Spermidine bindingBy similarity
Regioni302 – 3043Spermidine bindingBy similarity
Regioni311 – 3177Spermidine bindingBy similarity

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000228838.
InParanoidiQ6CG56.
KOiK00809.
OMAiDTYIDED.
OrthoDBiEOG73BVPW.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6CG56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETPNLATE AVLVHSAPVP EDAVPVSGID FDKPENKNTR AADLVGAMNG
60 70 80 90 100
MGFQATSLGQ ACNIIDEMRT WEGVDKEGNP AKTTIFLGYT SNLISSGLRS
110 120 130 140 150
TLKYLVKNKM VSAIVSSAGG IEEDLIKCLA ETYLGDFALK GSVLRETGMN
160 170 180 190 200
RIGNLLVPND NYCKFEEWVV PILDTMLEEQ EKQGVSWTPS TVIDRLGKEI
210 220 230 240 250
DNEDSVLYWA HKNQIPVFCP ALTDGSLGDM LFFHTFKASP LQLKIDIVAD
260 270 280 290 300
IRKINSMSMA ATRAGMIILG GGLVKHHIAN ACLMRNGADY AVYINTGQEF
310 320 330 340
DGSDAGARPD EAISWGKIKA GAKHVKVYAD ATLVFPLVVA ATFAKE
Length:346
Mass (Da):37,437
Last modified:August 16, 2004 - v1
Checksum:i3CF437D64712C3CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382128 Genomic DNA. Translation: CAG82570.1.
RefSeqiXP_500356.1. XM_500356.1.

Genome annotation databases

EnsemblFungiiCAG82570; CAG82570; YALI0_B00726g.
GeneIDi2907505.
KEGGiyli:YALI0B00726g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382128 Genomic DNA. Translation: CAG82570.1.
RefSeqiXP_500356.1. XM_500356.1.

3D structure databases

ProteinModelPortaliQ6CG56.
SMRiQ6CG56. Positions 5-346.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG82570; CAG82570; YALI0_B00726g.
GeneIDi2907505.
KEGGiyli:YALI0B00726g.

Phylogenomic databases

HOGENOMiHOG000228838.
InParanoidiQ6CG56.
KOiK00809.
OMAiDTYIDED.
OrthoDBiEOG73BVPW.

Enzyme and pathway databases

UniPathwayiUPA00354.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHYS_YARLI
AccessioniPrimary (citable) accession number: Q6CG56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: August 16, 2004
Last modified: November 11, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.