Reviewed, UniProtKB/Swiss-Prot Q6CEE7 (PPN1_YARLI)

Names and origin

Protein names

Recommended name:
    Endopolyphosphatase
    EC=3.6.1.10

Gene names

Name:	PPN1
Ordered Locus Names:	YALI0B16236g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of inorganic polyphosphate (poly P) chains of many hundreds of phosphate residues into shorter lengths By similarity.
Catalytic activity	Polyphosphate + n H2O = (n+1) oligophosphate.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein.
Post-translational modification	Processing by proteases in the vacuole may be required for activation By similarity.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endopolyphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 747	747	Endopolyphosphatase		PRO_0000058550
Regions
	Topological domain	1	1	Cytoplasmic Potential
	Transmembrane	2 – 22	21	Signal-anchor for type II membrane protein Potential
	Topological domain	23 – 747	725	Vacuolar Potential
	Compositional bias	591 – 624	34	Lys-rich
Amino acid modifications
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	463	1	N-linked (GlcNAc...) Potential
	Glycosylation	659	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6CEE7-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 9F6C5E25D8D75012	FASTA	747	85,087
	10 20 30 40 50 60 MLPKTLTIWA SLASLAVAQS GQVVFAKNAD GKFVHSTDDL DQGQRKIRGK FLHITDIHPD 70 80 90 100 110 120 PYFHVGAVAE DKCHVDPDHK DSDYPDEDSE LVWFRATGKK KHNHHKGKDH EKMPKAGYYG 130 140 150 160 170 180 HPLSSCDGPI SLMNATFDWI DQNIRDEIDF IIWTGDNVRH DNDNRYPRLE QDIFGYNQIV 190 200 210 220 230 240 SSKFHELFRY NETRDGEGHN GGGDPQHPLV IPIIPSLGNN DVFPHNLYLA GPSFQSRRML 250 260 270 280 290 300 QIWSEFVPEA QQHIFSRGSY YFQEVITGKL AVISLNTLYF YKSNPMSDGC DEKTDPGYKH 310 320 330 340 350 360 LVWLGVVLDE MRQRGMKVWL SGHVPPVEKN YEDSCHLKLA YWLTEYRDII VGSVFGHMNI 370 380 390 400 410 420 DHFVVMDPKK IEKAQSQDLG TPGLGYKSHV TDFLDVAISA SHPVHTFGSI YKRNYIESVR 430 440 450 460 470 480 EDYSEIPGPK KWLDEYASNF AIAHVSPSVI PNYFPSLRVW EYNITGLGEE IGNPPHPPPS 490 500 510 520 530 540 FRAWSDVLEE FERDYAQDVM DEIEVEWFDA NSDVIEAEDN DGDDDEDENE DDPEMLTEEA 550 560 570 580 590 600 IKEGVANINP ETGTLASIFG GLKFWKSSTA VATSSEPESD DYDSDLDAER KKGKKKGKKG 610 620 630 640 650 660 KKGKKGKKGK KKKGKKGKKG KKGKRDKSMP PKFPKDLQPG PAYIPQLFTP IGYTQYYANI 670 680 690 700 710 720 TQFNKEYKKT GASNFEYVVE YTTNDAPYNF EHLTVRNWVE LARVLGKNFR DLDLEAEKSK 730 740 SDQLWKVYMD RAFVGTGAEY LEEPDDD

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382128 Genomic DNA. Translation: CAG83218.1.
RefSeq	XP_500965.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2906836.
KEGG	yli:YALI0B16236g.
Phylogenomic databases
HOGENOM	Q6CEE7.
OMA	Q6CEE7. AISASHP.
Enzyme and pathway databases
BRENDA	3.6.1.10. 3602.
Family and domain databases
InterPro	IPR012358. EndopolyPtase_N1. IPR004843. M-pesterase. [Graphical view]
PANTHER	PTHR10340:SF4. EndopolyPtase_N1. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF027093. EndopolyPtase_N1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPN1_YARLI

Accession

Primary (citable) accession number: Q6CEE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)