Reviewed, UniProtKB/Swiss-Prot Q6CE88 (ERG27_YARLI)

Names and origin

Protein names

Recommended name:
    3-keto-steroid reductase
    EC=1.1.1.270

Gene names

Name:	ERG27
Ordered Locus Names:	YALI0B17644g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols By similarity.
Catalytic activity	4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP+ = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-keto-steroid reductase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	3-keto-steroid reductase		PRO_0000054594
Sites
	Active site	203	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CE88-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: A6A0233F4A782283	FASTA	343	38,724
	10 20 30 40 50 60 MIHNRKTQTV VITGASSNLG IAIGKRLIDE KKEDAHLTIV VTSRTLRNVR VAIKTLKAHA 70 80 90 100 110 120 VAKEVGPEVD FDYLLFDLAD MTSINGALVE LKLRFSRIDT LIFNSNAANY IGINWPLAMW 130 140 150 160 170 180 RFTTQFKSEI ENPSCMIQAV GVKSDDGMGS AYQSNVFGPW YMVLELTEQL KNGGKVIWIS 190 200 210 220 230 240 SITSSEKYVD LEDIELIHNK EPYKGSKRLI DVAHNYYSPK LEEEHGIYSY LTDPGIFTSS 250 260 270 280 290 300 SASEYLNIFS AFGMYLMFYF ARLIGLTTMN IDPYKGANVP VWVTLSEDPS ALKREYRLGS 310 320 330 340 RTGRWGTEMM DATKLQYEGS EEVGAYIDKG VGEWREKLKD QIN

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382128 Genomic DNA. Translation: CAG83277.1.
RefSeq	XP_501024.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CE88.
Genome annotation databases
GeneID	2907211.
GenomeReviews	Gene locus YALI0B17644g in contig CR382128_GR.
KEGG	yli:YALI0B17644g.
Phylogenomic databases
HOGENOM	Q6CE88.
OMA	EQYLVHP.
Enzyme and pathway databases
BRENDA	1.1.1.270. 3602.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

ERG27_YARLI

Accession

Primary (citable) accession number: Q6CE88

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)