Q6CE88 (ERG27_YARLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-keto-steroid reductase
EC=1.1.1.270

Gene names

Name:	ERG27
Ordered Locus Names:	YALI0B17644g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols By similarity.
Catalytic activity	4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP+ = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-keto sterol reductase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	3-keto-steroid reductase		PRO_0000054594
Sites
	Active site	203	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CE88 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: A6A0233F4A782283	FASTA	343	38,724
	10 20 30 40 50 60 MIHNRKTQTV VITGASSNLG IAIGKRLIDE KKEDAHLTIV VTSRTLRNVR VAIKTLKAHA 70 80 90 100 110 120 VAKEVGPEVD FDYLLFDLAD MTSINGALVE LKLRFSRIDT LIFNSNAANY IGINWPLAMW 130 140 150 160 170 180 RFTTQFKSEI ENPSCMIQAV GVKSDDGMGS AYQSNVFGPW YMVLELTEQL KNGGKVIWIS 190 200 210 220 230 240 SITSSEKYVD LEDIELIHNK EPYKGSKRLI DVAHNYYSPK LEEEHGIYSY LTDPGIFTSS 250 260 270 280 290 300 SASEYLNIFS AFGMYLMFYF ARLIGLTTMN IDPYKGANVP VWVTLSEDPS ALKREYRLGS 310 320 330 340 RTGRWGTEMM DATKLQYEGS EEVGAYIDKG VGEWREKLKD QIN

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382128 Genomic DNA. Translation: CAG83277.1.
RefSeq	XP_501024.1. XM_501024.1.
3D structure databases
ProteinModelPortal	Q6CE88.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CE88.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2907211.
GenomeReviews	Gene locus YALI0B17644g in contig CR382128_GR.
KEGG	yli:YALI0B17644g.
Phylogenomic databases
eggNOG	fuNOG05767.
HOGENOM	HBG398311.
OMA	VIWISSI.
OrthoDB	EOG4FBN2N.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K09827.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

ERG27_YARLI

Accession

Primary (citable) accession number: Q6CE88

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	August 16, 2004
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program