Q6CE48 (IND1_YARLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Iron-sulfur protein IND1

Alternative name(s):
Iron-sulfur protein required for NADH dehydrogenase 1

Gene names

Name:	IND1
Ordered Locus Names:	YALI0B18590g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia › Yarrowia lipolytica

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the effective assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). Probably facilitates the assembly of Fe-S cofactors and subunits of complex I. Ref.2
Cofactor	Binds 1 4Fe-4S cluster. Ref.2
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.2.
Sequence similarities	Belongs to the Mrp/NBP35 ATP-binding proteins family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Iron Iron-sulfur Metal-binding Nucleotide-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion Ref.2
	Chain	35 – 312	278	Iron-sulfur protein IND1		PRO_0000352527
Regions
	Nucleotide binding	75 – 82	8	ATP Potential
Experimental info
	Mutagenesis	242	1	C → A, E or S: Reduces the amount of fully assembled complex I. Ref.2
	Mutagenesis	245	1	C → A, E or S: Reduces the amount of fully assembled complex I. Ref.2
	Mutagenesis	279	1	C → A or S: No effect. Ref.2
	Mutagenesis	279	1	C → E: Abolishes most of complex I activity. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6CE48 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 25F5F96432C8A327	FASTA	312	33,167
	10 20 30 40 50 60 MRGFRLIAPI QRSIAIISRL QPITANFHSS PALRSHENPL GIPKSPASAP RIPRKTTRRP 70 80 90 100 110 120 EPIAGVKKTI VVSSAKGGVG KSTVSVNTAL SLAKRGLRVG LLDVDIFGPS IPTMFGLSGE 130 140 150 160 170 180 PRMTHEGKLI PMSKFGIQVM SMGFLVDPNK AVAWRGLLVQ KALEQLLQDV DWGTLDVLVM 190 200 210 220 230 240 DLPPGTGDVQ LTIAQTVKID GAIIVSTPQD VALVDVVRGL DLFEKTYTKV LGLVQNMSVF 250 260 270 280 290 300 VCPNCNHETH IFGVDGAVSK AKSRGLGVLG NVPLDPQICS QSDKGVPVAV SGGVQAKYYD 310 KIAEGVAEQL GV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.
[2]	"The iron-sulphur protein Ind1 is required for effective complex I assembly." Bych K., Kerscher S., Netz D.J.A., Pierik A.J., Zwicker K., Huynen M.A., Lill R., Brandt U., Balk J. EMBO J. 27:1736-1746(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 35-38, FUNCTION, COFACTOR, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-242; CYS-245 AND CYS-279.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382128 Genomic DNA. Translation: CAG83317.1.
RefSeq	XP_501064.1. XM_501064.1.
3D structure databases
ProteinModelPortal	Q6CE48.
ModBase	Search...
Protein-protein interaction databases
STRING	4952.Q6CE48.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2907193.
KEGG	yli:YALI0B18590g.
Phylogenomic databases
eggNOG	COG0489.
HOGENOM	HOG000079916.
KO	K03593.
OMA	PGQAIAW.
OrthoDB	EOG4PVS7G.
Family and domain databases
InterPro	IPR025669. AAA_dom. IPR019591. ATPase-like_ParA/MinD. IPR000808. Mrp_CS. [Graphical view]
Pfam	PF13614. AAA_31. 1 hit. PF10609. ParA. 1 hit. [Graphical view]
PROSITE	PS01215. MRP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IND1_YARLI

Accession

Primary (citable) accession number: Q6CE48

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program