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Q6CE12

- TRMB_YARLI

UniProt

Q6CE12 - TRMB_YARLI

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei110 – 1101S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei188 – 1881S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei191 – 1911UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    Transfer RNA methyltransferase 8UniRule annotation
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:TRM8UniRule annotation
    Ordered Locus Names:YALI0B19558g
    OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
    Taxonomic identifieri284591 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
    ProteomesiUP000001300: Chromosome B

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292tRNA (guanine-N(7)-)-methyltransferasePRO_0000370606Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with TRM82.UniRule annotation

    Protein-protein interaction databases

    STRINGi4952.Q6CE12.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6CE12.
    SMRiQ6CE12. Positions 73-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni133 – 1342S-adenosyl-L-methionine bindingUniRule annotation
    Regioni168 – 1692S-adenosyl-L-methionine bindingUniRule annotation
    Regioni266 – 2683S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000260965.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG708W9T.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6CE12-1 [UniParc]FASTAAdd to Basket

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    MLKRDQSEMD IEAETANMGK EEKESFVHKR QKYRQEQEEK RLAAKKGVSF    50
    EQPEDGEIKL PKKRYYRQRA HSNPFSDHQL EYPESPADMD WDVLYPNRKE 100
    GQKVEVADIG CGFGGLLVAL SPQLPNQLIL GMEIRVQVTN YVEDRIVALR 150
    HQNKETDGYQ NISVLRGNAM KFLPNFFEKG QLSKIFFCFP DPHFKQRKHK 200
    ARIVTTTLCS EYAYVVRPGG IVYTITDVKD LHEWMVKHLA AHPLFERLTK 250
    EEEDADPCVA TMLVETEEGK KVARNEGDKF VACFRRKENP EW 292
    Length:292
    Mass (Da):33,982
    Last modified:August 16, 2004 - v1
    Checksum:iEB71F2EF1F73C1D5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382128 Genomic DNA. Translation: CAG83353.1.
    RefSeqiXP_501100.1. XM_501100.1.

    Genome annotation databases

    EnsemblFungiiCAG83353; CAG83353; YALI0_B19558g.
    GeneIDi2907246.
    KEGGiyli:YALI0B19558g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382128 Genomic DNA. Translation: CAG83353.1 .
    RefSeqi XP_501100.1. XM_501100.1.

    3D structure databases

    ProteinModelPortali Q6CE12.
    SMRi Q6CE12. Positions 73-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4952.Q6CE12.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CAG83353 ; CAG83353 ; YALI0_B19558g .
    GeneIDi 2907246.
    KEGGi yli:YALI0B19558g.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000260965.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG708W9T.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Entry informationi

    Entry nameiTRMB_YARLI
    AccessioniPrimary (citable) accession number: Q6CE12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3