Reviewed, UniProtKB/Swiss-Prot Q6C8A3 (DNLI4_YARLI)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
Ordered Locus Names:	YALI0D21384g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	956 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 956	956	DNA ligase 4		PRO_0000278386
Regions
	Domain	700 – 793	94	BRCT 1
	Domain	857 – 956	100	BRCT 2
Sites
	Active site	309	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6C8A3-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 5169CFB807F4183B	FASTA	956	108,061
	10 20 30 40 50 60 MSSERRPELE ETAVDPATGS AASRKFSIVQ DAVETTIVAP TNHGPSPRFS TLVRNLFEPL 70 80 90 100 110 120 VNLSAVVAAL RKKPTEAKAH IASQFIKGWV EEVGKDIYPA FRLILPDKDR ERAVYGLKEK 130 140 150 160 170 180 ALGRLWVKVL NLAKDSPDAK ALSEWKQGGN ESAGNFSKRC YEVLSKRTSL TDYGHMTVDE 190 200 210 220 230 240 VNERLDLLAD GETDQAKQIE ILTYFYKHMN ATELKWLVNI ILRQMKMNAT EKVFFEPWHP 250 260 270 280 290 300 DAESLFNVTA SLKRVCWELT DPTKRLTSAE AQVSLFACFM PQIAAFPKYS GQDIAGKHFK 310 320 330 340 350 360 GRPFYIEEKI DGERMQMHMS EYGNKFHWWS RRSKDFTETY GNSLDDASGS LTKRLRGIIN 370 380 390 400 410 420 PKVRNCVLDG EMVAYDPATK KIIPFGTLRT ANRNEQNDLN LTKPMFMVFD ILLLNDKPLV 430 440 450 460 470 480 DYTLAERKRT LRTIFARTDN ETVGQEGVLE VLPYTEATTA AEIETCMRKI IAESSEGLVI 490 500 510 520 530 540 KDPTSVYRVN TRDDSWLKMK PEYMSEFGEK LDVVIIGGYY GSGKRGSILS SYLCGLRADG 550 560 570 580 590 600 SDQFWSFFKV GGGLTAGDYQ AIRTKTEGKW KRWDKNDKPK NVLLAGPNGD LERPDVWIEP 610 620 630 640 650 660 SDSVVVEVKA ASVVASDQYK VGLCLRFPRF RALRLDKTWE DGLTISQFAE LRQTVEMEAE 670 680 690 700 710 720 NKELELEDRK RRNAGPGRGA KRLKLANVSS DEDELGTDER PTSVFKATSF AVLSDMSSPR 730 740 750 760 770 780 YMSKAAVENL IKKHGGTVFQ TVEGPHTIPV ADTRTIKVQA LTKRVHGVDV IRPNWLLDCI 790 800 810 820 830 840 NEEKLVALEP RNLLESSAET LALAKTNVDE FGDSYTRPLT YKEMQEVLRF MDQFDLDQTN 850 860 870 880 890 900 PPDLMMEVLE TNDGAVPKGM LFYGKKVYMS TSNMDTVALE TQFRAYDALR CLQFGGANLV 910 920 930 940 950 TDMKDLVVAV AKTEEEAKEL RRVSSEQVFP FRVVSIKWVE ESWKNGTVEI EDDYPL

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382130 Genomic DNA. Translation: CAG81303.1.
RefSeq	XP_503109.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2911076.
KEGG	yli:YALI0D21384g.
Phylogenomic databases
HOGENOM	Q6C8A3.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_YARLI

Accession

Primary (citable) accession number: Q6C8A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)