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Q6C8A3 (DNLI4_YARLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 4

EC=6.5.1.1
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name:LIG4
Ordered Locus Names:YALI0D21384g
OrganismYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]
Taxonomic identifier284591 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia

Protein attributes

Sequence length956 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Ontologies

Keywords
   Biological processDNA damage
DNA recombination
DNA repair
DNA replication
   Cellular componentNucleus
   DomainRepeat
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA recombination

Inferred from electronic annotation. Source: UniProtKB-KW

DNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (ATP) activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 956956DNA ligase 4
PRO_0000278386

Regions

Domain700 – 79394BRCT 1
Domain857 – 956100BRCT 2

Sites

Active site3091N6-AMP-lysine intermediate By similarity
Metal binding3711Magnesium 1 Potential
Metal binding4761Magnesium 2 Potential
Binding site3071ATP By similarity
Binding site3141ATP By similarity
Binding site3311ATP By similarity
Binding site4811ATP By similarity
Binding site4921ATP By similarity
Binding site4981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6C8A3 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 5169CFB807F4183B

FASTA956108,061
        10         20         30         40         50         60 
MSSERRPELE ETAVDPATGS AASRKFSIVQ DAVETTIVAP TNHGPSPRFS TLVRNLFEPL 

        70         80         90        100        110        120 
VNLSAVVAAL RKKPTEAKAH IASQFIKGWV EEVGKDIYPA FRLILPDKDR ERAVYGLKEK 

       130        140        150        160        170        180 
ALGRLWVKVL NLAKDSPDAK ALSEWKQGGN ESAGNFSKRC YEVLSKRTSL TDYGHMTVDE 

       190        200        210        220        230        240 
VNERLDLLAD GETDQAKQIE ILTYFYKHMN ATELKWLVNI ILRQMKMNAT EKVFFEPWHP 

       250        260        270        280        290        300 
DAESLFNVTA SLKRVCWELT DPTKRLTSAE AQVSLFACFM PQIAAFPKYS GQDIAGKHFK 

       310        320        330        340        350        360 
GRPFYIEEKI DGERMQMHMS EYGNKFHWWS RRSKDFTETY GNSLDDASGS LTKRLRGIIN 

       370        380        390        400        410        420 
PKVRNCVLDG EMVAYDPATK KIIPFGTLRT ANRNEQNDLN LTKPMFMVFD ILLLNDKPLV 

       430        440        450        460        470        480 
DYTLAERKRT LRTIFARTDN ETVGQEGVLE VLPYTEATTA AEIETCMRKI IAESSEGLVI 

       490        500        510        520        530        540 
KDPTSVYRVN TRDDSWLKMK PEYMSEFGEK LDVVIIGGYY GSGKRGSILS SYLCGLRADG 

       550        560        570        580        590        600 
SDQFWSFFKV GGGLTAGDYQ AIRTKTEGKW KRWDKNDKPK NVLLAGPNGD LERPDVWIEP 

       610        620        630        640        650        660 
SDSVVVEVKA ASVVASDQYK VGLCLRFPRF RALRLDKTWE DGLTISQFAE LRQTVEMEAE 

       670        680        690        700        710        720 
NKELELEDRK RRNAGPGRGA KRLKLANVSS DEDELGTDER PTSVFKATSF AVLSDMSSPR 

       730        740        750        760        770        780 
YMSKAAVENL IKKHGGTVFQ TVEGPHTIPV ADTRTIKVQA LTKRVHGVDV IRPNWLLDCI 

       790        800        810        820        830        840 
NEEKLVALEP RNLLESSAET LALAKTNVDE FGDSYTRPLT YKEMQEVLRF MDQFDLDQTN 

       850        860        870        880        890        900 
PPDLMMEVLE TNDGAVPKGM LFYGKKVYMS TSNMDTVALE TQFRAYDALR CLQFGGANLV 

       910        920        930        940        950 
TDMKDLVVAV AKTEEEAKEL RRVSSEQVFP FRVVSIKWVE ESWKNGTVEI EDDYPL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR382130 Genomic DNA. Translation: CAG81303.1.
RefSeqXP_503109.1. XM_503109.1.

3D structure databases

ProteinModelPortalQ6C8A3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4952.Q6C8A3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCAG81303; CAG81303; YALI0_D21384g.
GeneID2911076.
KEGGyli:YALI0D21384g.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000176213.
KOK10777.
OMAKMDGDRM.
OrthoDBEOG7QC84G.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
InterProIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF00533. BRCT. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI4_YARLI
AccessionPrimary (citable) accession number: Q6C8A3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families