Reviewed, UniProtKB/Swiss-Prot Q6C842 (BUR1_YARLI)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase BUR1
    EC=2.7.11.22
    EC=2.7.11.23

Gene names

Name:	BUR1
Ordered Locus Names:	YALI0D22935g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in transcription regulation. Phosphorylates the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B and the silencing of telomeric-associated genes. Also required for histone H3 methylation. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Serine/threonine-protein kinase BUR1		PRO_0000085686
Regions
	Domain	38 – 339	302	Protein kinase
	Nucleotide binding	44 – 52	9	ATP By similarity
	Compositional bias	377 – 658	282	Pro-rich
	Compositional bias	387 – 706	320	Arg-rich
Sites
	Active site	168	1	Proton acceptor By similarity
	Binding site	67	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6C842-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 02C0A7D4A80049D2	FASTA	706	81,855
	10 20 30 40 50 60 MHGQPHQHLH TSPTAPFNMT IPYQPSTYYG CSNVTESYKP LGKIGEGTFG EVFRAEQITT 70 80 90 100 110 120 KRHVALKKIL LHSEKEGFPV TALREIRILK LLRHENVIPL VDLAVERGDQ SKKERGCVYM 130 140 150 160 170 180 VTPYMDHDLA GLLGNQSVQL SPAHIKCYML QLLEGIGYLH AKKFLHRDIK AANILVNDQG 190 200 210 220 230 240 ILKLADFGLA RGYDGPAPNS QTAGVNTENL TAMVVTRWYR PPELILGDRK YTTAIDMWGI 250 260 270 280 290 300 GCVFGEFFTR KPIFPGASDV DQGSKIFQAV GVPTEDTMPG WSVLPGAQNS NIWGTDATNK 310 320 330 340 350 360 LDKLFGRLSK DGLDFLKGLL LLDPTKRLTA IGGKNHAYFK TEPLPCQPHE LPKWQSSHEL 370 380 390 400 410 420 DNHKRREQEK NESKVQPPPR EKQPPVREPR DRDRSRDSRP PRERDSRDRR PPRGRYDSFE 430 440 450 460 470 480 RPERRPEGPR DGPRDRDRDE YPRELPPRDL PPRDLPPRDR DYPPRERDWD RRPPPRDRDY 490 500 510 520 530 540 PPRERDYPPR DSRDRDYHPR DRDYPPRDSR DYPRDSRDSR DSRPISPSRE RFHKRPQFDM 550 560 570 580 590 600 YSDTDYDRER DEDDRRRRPL PRDSSRDMTR DVYVPSRGRE PRELPKGPPP APGDKVEDKG 610 620 630 640 650 660 ESHNKDDKPC DTENPRDSDK SRDLGPPPGP PLPADGPPPP PSSNPPRPSE TYGGSRPPWR 670 680 690 700 RDSRDRRESR DRDGRDRDGR DRRLSSSRYA RDEFDEYGRK RPRIER

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382130 Genomic DNA. Translation: CAG81370.1.
RefSeq	XP_503170.1.
3D structure databases
SMR	Q6C842. Positions 40-353.
ModBase	Search...
Genome annotation databases
GeneID	2911247.
GenomeReviews	Gene locus YALI0D22935g in contig CR382130_GR.
KEGG	yli:YALI0D22935g.
Phylogenomic databases
eggNOG	fuNOG04620.
HOGENOM	HBG755340.
OMA	DRDYPPR.
OrthoDB	EOG96T4J6.
PhylomeDB	Q6C842.
Enzyme and pathway databases
BRENDA	2.7.11.22. 3602. 2.7.11.23. 3602.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUR1_YARLI

Accession

Primary (citable) accession number: Q6C842

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)