Q6C7U8 (PHO85_YARLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Negative regulator of the PHO system
EC=2.7.11.22

Alternative name(s):
Serine/threonine-protein kinase PHO85

Gene names

Name:	PHO85
Ordered Locus Names:	YALI0D25190g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia › Yarrowia lipolytica

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	When phosphate concentrations are high it phosphorylates the PHO4 transcription factor thus establishing repression By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with a number of cyclins By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Negative regulator of the PHO system		PRO_0000086520
Regions
	Domain	7 – 289	283	Protein kinase
	Nucleotide binding	13 – 21	9	ATP By similarity
Sites
	Active site	130	1	Proton acceptor By similarity
	Binding site	36	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6C7U8 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 5764A684447E6C79	FASTA	294	33,671
	10 20 30 40 50 60 MNTSSQFQQL EKLGEGTYAT VYKGRNRTTG QLVALKEINL DSEEGTPSTA IREISLMKEL 70 80 90 100 110 120 KHENIVTLYD VIHTENKLNL VFEYMDKDLK KFMDTNGNKG ALETKQVKWF MYQLLRGILF 130 140 150 160 170 180 CHDNRVLHRD LKPQNLLINA KGQLKLADFG LARAFGIPVN TFSNEVVTLW YRAPDVLLGS 190 200 210 220 230 240 RTYSTSIDIW SAGCIMAEMF TGRPLFPGSS NDDQLQHIFK LMGTPNESTW PNISSLPNYR 250 260 270 280 290 SNFQVYAPQD LRVIIPQIDN VALDLLLSLL QLKPENRITA RQSLEHPWFA EYHQ

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382130 Genomic DNA. Translation: CAG81468.1.
RefSeq	XP_503264.1. XM_503264.1.
3D structure databases
ProteinModelPortal	Q6C7U8.
SMR	Q6C7U8. Positions 7-293.
ModBase	Search...
Protein-protein interaction databases
STRING	4952.Q6C7U8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2910782.
KEGG	yli:YALI0D25190g.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K06655.
OMA	FRAYNFT.
OrthoDB	EOG4QJVX0.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHO85_YARLI

Accession

Primary (citable) accession number: Q6C7U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program