Reviewed, UniProtKB/Swiss-Prot Q6C7G2 (NMT_YARLI)

Names and origin

Protein names

Recommended name:
    Glycylpeptide N-tetradecanoyltransferase
    EC=2.3.1.97
Alternative name(s):
    Peptide N-myristoyltransferase
    Myristoyl-CoA:protein N-myristoyltransferase
      Short name=NMT

Gene names

Name:	NMT1
Ordered Locus Names:	YALI0E01078g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Glycylpeptide N-tetradecanoyltransferase		PRO_0000064246
Regions
	Region	34 – 37	4	Myristoyl CoA-binding By similarity
	Region	164 – 200	37	Myristoyl CoA-binding By similarity
Sites
	Active site	443	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6C7G2-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 6F53890F089E579B	FASTA	443	51,097
	10 20 30 40 50 60 MSADKFNEIL KMFQQQMAMQ DIPDREKAKK WEEYLFWKTQ PVPKFDEDID SEGPIEHKKL 70 80 90 100 110 120 EDVRPTPYNL PAEYEWSDVD IENPEHIQEV YDLLYDNYVE DDDATFRFKY SASFLDWALK 130 140 150 160 170 180 PPGWQKTWYP CVRVAATGKM VAFISAIPTS IQLRDNDLIK AVEINFLCVH KKLRSKRLAP 190 200 210 220 230 240 VLIKEITRRV NQKDIWQALY TAGVVLPSPV STCRYYHRPL NWTKLYEIGF SAIPPNQTAA 250 260 270 280 290 300 SMVARYTLPK EVPLKVRAMT KADVKAVHAI LQNYLSAASE MSQHFSEEEV AHWFVDPISE 310 320 330 340 350 360 DKNDDKIVYS YVVEEDGKVI DFFSFYKLDH TVLKEWATES NLRAAYGFYY ATSSLSSAKA 370 380 390 400 410 420 RQDRVKELIG SAVILANNLG FEVYNELTLL DNPSHLDDLK FGCGDGYLNY YLFNYRAQPV 430 440 HGGLDKQKQL EQLDGKGVGV IML

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382131 Genomic DNA. Translation: CAG78979.1.
RefSeq	XP_503400.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6C7G2.
Genome annotation databases
GeneID	2912937.
GenomeReviews	Gene locus YALI0E01078g in contig CR382131_GR.
KEGG	yli:YALI0E01078g.
Phylogenomic databases
HOGENOM	Q6C7G2.
OMA	VEDDDSM.
Enzyme and pathway databases
BRENDA	2.3.1.97. 3602.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. Myristoyl_trans. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT_YARLI

Accession

Primary (citable) accession number: Q6C7G2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)