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Q6C4Y5

- DOT1_YARLI

UniProt

Q6C4Y5 - DOT1_YARLI

Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

DOT1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

    Enzyme regulationi

    Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei346 – 3461S-adenosyl-L-methioninePROSITE-ProRule annotation

    GO - Molecular functioni

    1. histone-lysine N-methyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC:2.1.1.43)
    Alternative name(s):
    Histone H3-K79 methyltransferase
    Short name:
    H3-K79-HMTase
    Gene namesi
    Name:DOT1
    Ordered Locus Names:YALI0E22715g
    OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
    Taxonomic identifieri284591 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
    ProteomesiUP000001300: Chromosome E

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Histone-lysine N-methyltransferase, H3 lysine-79 specificPRO_0000270617Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi4952.Q6C4Y5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6C4Y5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini168 – 491324DOT1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni297 – 3004S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
    Regioni320 – 32910S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
    Regioni382 – 3832S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.PROSITE-ProRule annotation
    Contains 1 DOT1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG294902.
    HOGENOMiHOG000176257.
    KOiK11427.
    OMAiATSIHIT.
    OrthoDBiEOG7KH9VN.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR013110. DOT1.
    IPR025789. Histone_H3-K79_MeTrfase.
    IPR021162. Histone_H3-K79_MeTrfase_fungi.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF08123. DOT1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51569. DOT1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6C4Y5-1 [UniParc]FASTAAdd to Basket

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    MFFSHLGKKN GGPTAKSSDV KRKVTKVKTR KPVVTSVSKS PSPSKTAPPP    50
    ATAAAAATAA AKPSTPRKSR RKTPNIYQLS KSSLSEVDTE ESEREVSSSL 100
    STPGLYELVP RDIVTAGFGS AELATSIHIT NASESGNLSM TDIYEPVSTD 150
    KSLSDRVKLG ALSCDFVEDY SLIKPKVPGE FEPVREILSI MEMTALHFVD 200
    KGASEEIKHP VMDDCIMRRF RRSYEGGDLE GMKTSMKEFD EVVKTQRAEG 250
    AILANLKQLT AVPQDLAYFL LNQVYSRIVS PESKSLRDYK AFSNNVYGEL 300
    MPPFMSTVFQ KTDLQPSSVF VDLGSGVGNC TLQAALEVGC ESWGCEVMTN 350
    ASSLAEKQKI ELYSRAKMFG IKTGDIHLVA SSFVHNDEVH SAISRADVLL 400
    VNNYAFDGTL NAHLLDMFLD LKEGCKIVSL KSFVPVGHVI SEHNIESPVN 450
    ILKVQKLDFY SGSVSWTAAG GTYYISTVDR SAIKAFLSKG GY 492
    Length:492
    Mass (Da):53,624
    Last modified:August 16, 2004 - v1
    Checksum:i32609F4429A5D9F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382131 Genomic DNA. Translation: CAG79874.1.
    RefSeqiXP_504277.1. XM_504277.1.

    Genome annotation databases

    EnsemblFungiiCAG79874; CAG79874; YALI0_E22715g.
    GeneIDi2912906.
    KEGGiyli:YALI0E22715g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382131 Genomic DNA. Translation: CAG79874.1 .
    RefSeqi XP_504277.1. XM_504277.1.

    3D structure databases

    ProteinModelPortali Q6C4Y5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4952.Q6C4Y5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CAG79874 ; CAG79874 ; YALI0_E22715g .
    GeneIDi 2912906.
    KEGGi yli:YALI0E22715g.

    Phylogenomic databases

    eggNOGi NOG294902.
    HOGENOMi HOG000176257.
    KOi K11427.
    OMAi ATSIHIT.
    OrthoDBi EOG7KH9VN.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR013110. DOT1.
    IPR025789. Histone_H3-K79_MeTrfase.
    IPR021162. Histone_H3-K79_MeTrfase_fungi.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF08123. DOT1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51569. DOT1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Entry informationi

    Entry nameiDOT1_YARLI
    AccessioniPrimary (citable) accession number: Q6C4Y5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3