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Q6C4Y5 (DOT1_YARLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific

EC=2.1.1.43
Alternative name(s):
Histone H3-K79 methyltransferase
Short name=H3-K79-HMTase
Gene names
Name:DOT1
Ordered Locus Names:YALI0E22715g
OrganismYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]
Taxonomic identifier284591 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Enzyme regulation

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 By similarity.

Subcellular location

Nucleus By similarity.

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

Contains 1 DOT1 domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
   LigandS-adenosyl-L-methionine
   Molecular functionChromatin regulator
Methyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhistone-lysine N-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Histone-lysine N-methyltransferase, H3 lysine-79 specific
PRO_0000270617

Regions

Domain168 – 491324DOT1
Region297 – 3004S-adenosyl-L-methionine binding By similarity
Region320 – 32910S-adenosyl-L-methionine binding By similarity
Region382 – 3832S-adenosyl-L-methionine binding By similarity

Sites

Binding site3461S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6C4Y5 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 32609F4429A5D9F6

FASTA49253,624
        10         20         30         40         50         60 
MFFSHLGKKN GGPTAKSSDV KRKVTKVKTR KPVVTSVSKS PSPSKTAPPP ATAAAAATAA 

        70         80         90        100        110        120 
AKPSTPRKSR RKTPNIYQLS KSSLSEVDTE ESEREVSSSL STPGLYELVP RDIVTAGFGS 

       130        140        150        160        170        180 
AELATSIHIT NASESGNLSM TDIYEPVSTD KSLSDRVKLG ALSCDFVEDY SLIKPKVPGE 

       190        200        210        220        230        240 
FEPVREILSI MEMTALHFVD KGASEEIKHP VMDDCIMRRF RRSYEGGDLE GMKTSMKEFD 

       250        260        270        280        290        300 
EVVKTQRAEG AILANLKQLT AVPQDLAYFL LNQVYSRIVS PESKSLRDYK AFSNNVYGEL 

       310        320        330        340        350        360 
MPPFMSTVFQ KTDLQPSSVF VDLGSGVGNC TLQAALEVGC ESWGCEVMTN ASSLAEKQKI 

       370        380        390        400        410        420 
ELYSRAKMFG IKTGDIHLVA SSFVHNDEVH SAISRADVLL VNNYAFDGTL NAHLLDMFLD 

       430        440        450        460        470        480 
LKEGCKIVSL KSFVPVGHVI SEHNIESPVN ILKVQKLDFY SGSVSWTAAG GTYYISTVDR 

       490 
SAIKAFLSKG GY 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR382131 Genomic DNA. Translation: CAG79874.1.
RefSeqXP_504277.1. XM_504277.1.

3D structure databases

ProteinModelPortalQ6C4Y5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4952.Q6C4Y5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCAG79874; CAG79874; YALI0_E22715g.
GeneID2912906.
KEGGyli:YALI0E22715g.

Phylogenomic databases

eggNOGNOG294902.
HOGENOMHOG000176257.
KOK11427.
OMAATSIHIT.
OrthoDBEOG7KH9VN.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR021162. Histone_H3-K79_MeTrfase_fungi.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51569. DOT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDOT1_YARLI
AccessionPrimary (citable) accession number: Q6C4Y5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: August 16, 2004
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families