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Q6C4Y5

- DOT1_YARLI

UniProt

Q6C4Y5 - DOT1_YARLI

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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

DOT1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Enzyme regulationi

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei346 – 3461S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

  1. histone-lysine N-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC:2.1.1.43)
Alternative name(s):
Histone H3-K79 methyltransferase
Short name:
H3-K79-HMTase
Gene namesi
Name:DOT1
Ordered Locus Names:YALI0E22715g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
ProteomesiUP000001300: Chromosome E

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Histone-lysine N-methyltransferase, H3 lysine-79 specificPRO_0000270617Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi4952.Q6C4Y5.

Structurei

3D structure databases

ProteinModelPortaliQ6C4Y5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini168 – 491324DOT1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni297 – 3004S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni320 – 32910S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni382 – 3832S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.PROSITE-ProRule annotation
Contains 1 DOT1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG294902.
HOGENOMiHOG000176257.
InParanoidiQ6C4Y5.
KOiK11427.
OMAiATSIHIT.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR021162. Histone_H3-K79_MeTrfase_fungi.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6C4Y5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFFSHLGKKN GGPTAKSSDV KRKVTKVKTR KPVVTSVSKS PSPSKTAPPP
60 70 80 90 100
ATAAAAATAA AKPSTPRKSR RKTPNIYQLS KSSLSEVDTE ESEREVSSSL
110 120 130 140 150
STPGLYELVP RDIVTAGFGS AELATSIHIT NASESGNLSM TDIYEPVSTD
160 170 180 190 200
KSLSDRVKLG ALSCDFVEDY SLIKPKVPGE FEPVREILSI MEMTALHFVD
210 220 230 240 250
KGASEEIKHP VMDDCIMRRF RRSYEGGDLE GMKTSMKEFD EVVKTQRAEG
260 270 280 290 300
AILANLKQLT AVPQDLAYFL LNQVYSRIVS PESKSLRDYK AFSNNVYGEL
310 320 330 340 350
MPPFMSTVFQ KTDLQPSSVF VDLGSGVGNC TLQAALEVGC ESWGCEVMTN
360 370 380 390 400
ASSLAEKQKI ELYSRAKMFG IKTGDIHLVA SSFVHNDEVH SAISRADVLL
410 420 430 440 450
VNNYAFDGTL NAHLLDMFLD LKEGCKIVSL KSFVPVGHVI SEHNIESPVN
460 470 480 490
ILKVQKLDFY SGSVSWTAAG GTYYISTVDR SAIKAFLSKG GY
Length:492
Mass (Da):53,624
Last modified:August 16, 2004 - v1
Checksum:i32609F4429A5D9F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382131 Genomic DNA. Translation: CAG79874.1.
RefSeqiXP_504277.1. XM_504277.1.

Genome annotation databases

EnsemblFungiiCAG79874; CAG79874; YALI0_E22715g.
GeneIDi2912906.
KEGGiyli:YALI0E22715g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382131 Genomic DNA. Translation: CAG79874.1 .
RefSeqi XP_504277.1. XM_504277.1.

3D structure databases

ProteinModelPortali Q6C4Y5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4952.Q6C4Y5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CAG79874 ; CAG79874 ; YALI0_E22715g .
GeneIDi 2912906.
KEGGi yli:YALI0E22715g.

Phylogenomic databases

eggNOGi NOG294902.
HOGENOMi HOG000176257.
InParanoidi Q6C4Y5.
KOi K11427.
OMAi ATSIHIT.
OrthoDBi EOG7KH9VN.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR021162. Histone_H3-K79_MeTrfase_fungi.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF08123. DOT1. 1 hit.
[Graphical view ]
PIRSFi PIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51569. DOT1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Entry informationi

Entry nameiDOT1_YARLI
AccessioniPrimary (citable) accession number: Q6C4Y5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: August 16, 2004
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3