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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

DOT1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones (By similarity).By similarity

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Enzyme regulationi

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei346S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Methyltransferase, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC:2.1.1.43)
Alternative name(s):
Histone H3-K79 methyltransferase
Short name:
H3-K79-HMTase
Gene namesi
Name:DOT1
Ordered Locus Names:YALI0E22715g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
Proteomesi
  • UP000001300 Componenti: Chromosome E

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002706171 – 492Histone-lysine N-methyltransferase, H3 lysine-79 specificAdd BLAST492

Proteomic databases

PRIDEiQ6C4Y5

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi4952.XP_504277.1

Structurei

3D structure databases

ProteinModelPortaliQ6C4Y5
SMRiQ6C4Y5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 491DOT1PROSITE-ProRule annotationAdd BLAST324

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni297 – 300S-adenosyl-L-methionine bindingPROSITE-ProRule annotation4
Regioni320 – 329S-adenosyl-L-methionine bindingPROSITE-ProRule annotation10
Regioni382 – 383S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000176257
InParanoidiQ6C4Y5
KOiK11427
OMAiINRRTIN
OrthoDBiEOG092C18VU

Family and domain databases

InterProiView protein in InterPro
IPR021162 Dot1
IPR025789 DOT1_dom
IPR030445 H3-K79_meTrfase
IPR029063 SAM-dependent_MTases
PANTHERiPTHR21451 PTHR21451, 2 hits
PfamiView protein in Pfam
PF08123 DOT1, 1 hit
PIRSFiPIRSF017570 Histone_H3-K79_MeTrfase, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51569 DOT1, 1 hit

Sequencei

Sequence statusi: Complete.

Q6C4Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFFSHLGKKN GGPTAKSSDV KRKVTKVKTR KPVVTSVSKS PSPSKTAPPP
60 70 80 90 100
ATAAAAATAA AKPSTPRKSR RKTPNIYQLS KSSLSEVDTE ESEREVSSSL
110 120 130 140 150
STPGLYELVP RDIVTAGFGS AELATSIHIT NASESGNLSM TDIYEPVSTD
160 170 180 190 200
KSLSDRVKLG ALSCDFVEDY SLIKPKVPGE FEPVREILSI MEMTALHFVD
210 220 230 240 250
KGASEEIKHP VMDDCIMRRF RRSYEGGDLE GMKTSMKEFD EVVKTQRAEG
260 270 280 290 300
AILANLKQLT AVPQDLAYFL LNQVYSRIVS PESKSLRDYK AFSNNVYGEL
310 320 330 340 350
MPPFMSTVFQ KTDLQPSSVF VDLGSGVGNC TLQAALEVGC ESWGCEVMTN
360 370 380 390 400
ASSLAEKQKI ELYSRAKMFG IKTGDIHLVA SSFVHNDEVH SAISRADVLL
410 420 430 440 450
VNNYAFDGTL NAHLLDMFLD LKEGCKIVSL KSFVPVGHVI SEHNIESPVN
460 470 480 490
ILKVQKLDFY SGSVSWTAAG GTYYISTVDR SAIKAFLSKG GY
Length:492
Mass (Da):53,624
Last modified:August 16, 2004 - v1
Checksum:i32609F4429A5D9F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382131 Genomic DNA Translation: CAG79874.1
RefSeqiXP_504277.1, XM_504277.1

Genome annotation databases

EnsemblFungiiCAG79874; CAG79874; YALI0_E22715g
GeneIDi2912906
KEGGiyli:YALI0E22715g

Similar proteinsi

Entry informationi

Entry nameiDOT1_YARLI
AccessioniPrimary (citable) accession number: Q6C4Y5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: August 16, 2004
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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