Q6C3K2 (ALG1_YARLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase
EC=2.4.1.142

Alternative name(s):
Asparagine-linked glycosylation protein 1
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase

Gene names

Name:	ALG1
Ordered Locus Names:	YALI0E34133g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER By similarity.
Catalytic activity	GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitobiosyldiphosphodolichol beta-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Chitobiosyldiphosphodolichol beta-mannosyltransferase		PRO_0000080258
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	106 – 126	21	Helical; Potential
	Transmembrane	189 – 209	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6C3K2 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 18D986BA71EBF758	FASTA	463	52,711
	10 20 30 40 50 60 MKAWHWSVTL VVIYLAIPVI LYLLTRKDDR KPLSDIRKRK RTIVLVLGDL GRSPRMLYHA 70 80 90 100 110 120 RSLARSGHKV DLCGYDGAKP FDEILNNDLI KIHHIPLILN TRKLPFVVFG ILKVIRQHWL 130 140 150 160 170 180 LISLLYKLRG ADYLLVQNPP SIPTLGVVRF YNLFLSTRTK VVLDWHNFGY TILALKLPET 190 200 210 220 230 240 HPMVKFAKFY EGFFGGRAFV HLCVTVLMGQ AMRKTFGMSG RRIVPLHDRP AFHFKPLSES 250 260 270 280 290 300 EKLDVLRDFK ETLYDDMTAD HKIIVSSTSY TPDENFNILL DALALYDESK LDLPPLRVII 310 320 330 340 350 360 TGKGPMMPEF LAKVEKLQLK RVSIRTAWLE FADYPRILGA AHLGVSLHES SSGYDLPMKV 370 380 390 400 410 420 VDMFGCGIPV VSVDYAALSE LVKTNTNGVA VKGHVEMGNT FMSLFSNRGK LDNIKRGAMI 430 440 450 460 ESRNTWDQTW VKTVGPLFDI GEYVQQRPDE DYDFSSSSSD DDH

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382131 Genomic DNA. Translation: CAG80366.1.
RefSeq	XP_504760.1. XM_504760.1.
3D structure databases
ProteinModelPortal	Q6C3K2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6C3K2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2911951.
GenomeReviews	Gene locus YALI0E34133g in contig CR382131_GR.
KEGG	yli:YALI0E34133g.
Phylogenomic databases
eggNOG	fuNOG05192.
HOGENOM	HBG319770.
OMA	LQNPPGL.
OrthoDB	EOG4WWVT5.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
KO	K03842.
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG1_YARLI

Accession

Primary (citable) accession number: Q6C3K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 16, 2004
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program