Reviewed, UniProtKB/Swiss-Prot Q6C2N7 (ATG15_YARLI)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	ATG15
Ordered Locus Names:	YALI0F06358g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Putative lipase ATG15		PRO_0000090371
Regions
	Topological domain	1 – 19	19	Cytoplasmic Potential
	Transmembrane	20 – 42	23	Signal-anchor for type II membrane protein Potential
	Topological domain	43 – 549	507	Lumenal Potential
Sites
	Active site	331	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Glycosylation	448	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6C2N7-1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 1B426DD67E8D4298	FASTA	549	61,062
	10 20 30 40 50 60 MKQDLYKESS PPPSTTKSKG LYVIVAALVT TAIYLLYSQG YSNTHGEKDM PSVVPNLVLP 70 80 90 100 110 120 ANPSSDHSFA VKHIYHHNTE ADANHGRMDV SGEWVRAAQK AQHLNLGQDT HRYMASNARD 130 140 150 160 170 180 PYTNLPLKSR TQKVKRWRQR DPDHVESYLE AARLNPQLYG AMDFDWVEED ILVPDVTDRD 190 200 210 220 230 240 TVVSLAVMAS NAYVDVPFTG DWTNVSWKET GGIGWQSDGV RGHIFVDQTP GSPLVVIALK 250 260 270 280 290 300 GTSAAIFDSG GDTVINDKTN DNLLFSCCCA RVSYLWNTVC DCYTGESYTC DQECLEKELY 310 320 330 340 350 360 AEDRYYRAVL DIYRNVTHLY PQKQIWVTGH SLGGALSAML GRTYGIPAVG YEAPGELLPT 370 380 390 400 410 420 KRLHLPSPPG IPWSQEHIWH FGHTADPIFM GVCNGASSSC SIGGYAMETS CHSGLQCMYD 430 440 450 460 470 480 VVTDKGWHLS MVNHRIHTVI DEVLLAYNET AACVPPPPCQ DCFNWNFVMG NDKDDDDKDK 490 500 510 520 530 540 KKKKKTSTSS SVVSKTKTST SSTVATNTMP SLPDPTCVER NWYGKCIRYD PEIKQQYGDS HTVTHVTMA

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382132 Genomic DNA. Translation: CAG77882.2.
RefSeq	XP_505075.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2908407.
GenomeReviews	Gene locus YALI0F06358g in contig CR382132_GR.
KEGG	yli:YALI0F06358g.
Phylogenomic databases
HOGENOM	Q6C2N7.
Enzyme and pathway databases
BRENDA	3.1.1.3. 3602.
Family and domain databases
InterPro	IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_YARLI

Accession

Primary (citable) accession number: Q6C2N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	October 13, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)