Q6C2H4 (CET1_YARLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA-capping enzyme subunit beta
EC=3.1.3.33

Alternative name(s):
Polynucleotide 5'-triphosphatase
mRNA 5'-triphosphatase
Short name=TPase

Gene names

Name:	CET1
Ordered Locus Names:	YALI0F07865g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end By similarity.
Catalytic activity	A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
Cofactor	Divalent ions By similarity.
Subunit structure	The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the fungal TPase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	mRNA-capping enzyme subunit beta		PRO_0000210118
Regions
	Compositional bias	61 – 66	6	Poly-Asp

Sequences

	Sequence		Length	Mass (Da)
	Q6C2H4 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 09186169A060433C	FASTA	386	43,288
	10 20 30 40 50 60 MDIGKMINDD NGSDSRRTSV KSLLNSPPTG LPSSDDRPDS KASSTSDLAQ QLTNEMESGE 70 80 90 100 110 120 DDDDEDAAAA VNHKPRRYSR PPIWATKWQG TGRHAERDHR PPPHRQDRRD PRMERQSGGS 130 140 150 160 170 180 RALPASSTTT ITGCPPSISG IKPFESVTRT VTSWLHAHLS TMSPEQLQTV ELEAKIGTIQ 190 200 210 220 230 240 HKKAGADRAR LDLPIVTEAV VNQQYVQAQC SFSSQLPESL LEEAKRILDA ADPKFIKSTE 250 260 270 280 290 300 HTIHRDEIYE GQQDKGNLRI TRDDVTGRQV AKIRKKAIAH IMIHCPTDPF DIRLSLATES 310 320 330 340 350 360 PTDDVPQGVC RTRRKDRISY LYDGFRADLT KVSGSSMSSE LEMEADSHKL IGYFTDRNDP 370 380 HNMDKVEELL QILLDSMRYV NRRLKA

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382132 Genomic DNA. Translation: CAG77945.1.
RefSeq	XP_505138.1. XM_505138.1.
3D structure databases
ProteinModelPortal	Q6C2H4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2908374.
GenomeReviews	Gene locus YALI0F07865g in contig CR382132_GR.
KEGG	yli:YALI0F07865g.
Phylogenomic databases
eggNOG	fuNOG08284.
OMA	MSSELEM.
OrthoDB	EOG4N605N.
Family and domain databases
InterPro	IPR023577. CYTH-like_domain. IPR004206. mRNA_capping_enz_bsu_dom. [Graphical view]
Gene3D	G3DSA:3.20.100.10. mRNA_capping_enz_bsu. 1 hit.
Pfam	PF02940. mRNA_triPase. 1 hit. [Graphical view]
SUPFAM	SSF55154. mRNA_capping_enz_bsu. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CET1_YARLI

Accession

Primary (citable) accession number: Q6C2H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 16, 2004
Last modified:	December 14, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program