Reviewed, UniProtKB/Swiss-Prot Q6C2A3 (BUD32_YARLI)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase BUD32
    EC=2.7.11.1

Gene names

Name:	BUD32
Ordered Locus Names:	YALI0F09471g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation By similarity. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Component of the EKC/KEOPS By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Telomere By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosomal protein Cytoplasm Nucleus Telomere
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Serine/threonine-protein kinase BUD32		PRO_0000278918
Regions
	Domain	12 – 248	237	Protein kinase
	Nucleotide binding	18 – 26	9	ATP By similarity
Sites
	Active site	159	1	Proton acceptor By similarity
	Binding site	42	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6C2A3-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 7CE7D43CED05AF8C	FASTA	248	27,804
	10 20 30 40 50 60 MITLPEQLEK LSKQENLIAQ GAESLVYSAQ HPYLPQQECI VKYRPKKPYR LPELDAQLSK 70 80 90 100 110 120 HRTLAEARVL QKLALGDVEV PHLVFIDAKN GLIYMEKIEG LSVKQWIWNE EGDTEGGAQE 130 140 150 160 170 180 AGDKSRSLPD GDVSSLKDTL VLVGQEIGKL HKSDIVHGDL TTSNVMLRDG KPVIIDFGLA 190 200 210 220 230 240 SVSTLAEDKA VDLYVMERAV LSTHPVHSQQ YCDWLFEGYL AVVGKSQKEV MRKLEDVRQR GRKRSMLG

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382132 Genomic DNA. Translation: CAG78016.1.
RefSeq	XP_505209.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2908942.
KEGG	yli:YALI0F09471g.
Phylogenomic databases
HOGENOM	Q6C2A3.
OMA	Q6C2A3. VVYFVDY.
Enzyme and pathway databases
BRENDA	2.7.11.1. 3602.
Family and domain databases
InterPro	IPR002575. APH_trans. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR008266. Tyr_pkinase_AS. [Graphical view]
Pfam	PF01636. APH. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUD32_YARLI

Accession

Primary (citable) accession number: Q6C2A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)