Q6C1W9 (TRM10_YARLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(9)-N1)-methyltransferase
EC=2.1.1.221

Alternative name(s):
tRNA methyltransferase 10
tRNA(m1G9)-methyltransferase
Short name=tRNA(m1G9)MTase

Gene names

Name:	TRM10
Ordered Locus Names:	YALI0F12771g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine9 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine9 in tRNA.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	tRNA (guanine(9)-N1)-methyltransferase		PRO_0000060519
Regions
	Compositional bias	318 – 368	51	Glu-rich

Sequences

	Sequence		Length	Mass (Da)
	Q6C1W9 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: DB7E85996247F4CB	FASTA	371	41,649
	10 20 30 40 50 60 MVDVTEPVAP VEAVKETAGA SSATPEVDRT SEPHPKNPQN VPAPYNTKTA VIPEGMSKNE 70 80 90 100 110 120 WKKAQKKAIW ESKKDEIAAV KKEKKKAARK RKQLAISRGE IPAPIPQDER PPQTQLPISI 130 140 150 160 170 180 VLDCGFDEMM TQKEKVSLSA QVGRCYSANR KADARFDLTV NSFNKGLKDR FNNEMNKVHE 190 200 210 220 230 240 LWTNIKFVED DYTVPEDETA KSKLVYLSSD SDNVINELED GKTYIIGGIV DKGRYKNLCQ 250 260 270 280 290 300 DKASKQGLQT GRLPIADFIK LSGRKVLTTN HVFEILLKWT ELKDWKAAFE AVLPMRKLDP 310 320 330 340 350 360 ANYGRAHRRA RKKARLAEGA EGNDEEDDED DDDEEEEEEE SVSAEPEVVE TKAEVETDTE 370 TKAETEAETK A

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382132 Genomic DNA. Translation: CAG78150.1.
RefSeq	XP_505343.1. XM_505343.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6C1W9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2908201.
GenomeReviews	Gene locus YALI0F12771g in contig CR382132_GR.
KEGG	yli:YALI0F12771g.
Phylogenomic databases
eggNOG	fuNOG04600.
HOGENOM	HBG717479.
OMA	GAFVDHN.
OrthoDB	EOG48D43Z.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. [Graphical view]
KO	K15445.
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRM10_YARLI

Accession

Primary (citable) accession number: Q6C1W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program