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Protein

Enolase

Gene

ENO

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GPD)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase (YALI0_B02728g)
  4. Enolase (ENO)
  5. Pyruvate kinase (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateBy similarity1
Binding sitei168SubstrateBy similarity1
Active sitei211Proton donorBy similarity1
Metal bindingi246MagnesiumBy similarity1
Metal bindingi297MagnesiumBy similarity1
Binding sitei297SubstrateBy similarity1
Metal bindingi322MagnesiumBy similarity1
Binding sitei322SubstrateBy similarity1
Active sitei347Proton acceptorBy similarity1
Binding sitei398SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENO
Ordered Locus Names:YALI0F16819g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
Proteomesi
  • UP000001300 Componenti: Chromosome F

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340611 – 438EnolaseAdd BLAST438

Proteomic databases

PRIDEiQ6C1F3.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6C1F3.
SMRiQ6C1F3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni374 – 377Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiQ6C1F3.
KOiK01689.
OMAiSKWGGKG.
OrthoDBiEOG092C2W5X.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6C1F3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVEKLHARY VYDSRGNPTV EVDLTTQHGL FRAIVPSGAS TGVHEALELR
60 70 80 90 100
DKDASKWGGK GVLKAVQNVN EIIAPAVIDA KLDVKDQAAF DKFLLELDGT
110 120 130 140 150
ENKSKLGANA ILGVSIAAAR AGAGEKGVPL YEHIASLASS PQPYVLPVPF
160 170 180 190 200
LNVLNGGSHA GGRLAIQEFM IAPTEFESFS ESLRAGTEVY HELKKLAKKE
210 220 230 240 250
YGASAGNVGD EGGVAPDIQT AEEALNLITE AIDAAGYTGK IKIAIDAASS
260 270 280 290 300
EFYNEEANKY DLDFKNPDSD KSLWKTGEEL AAYYSELIKK YPIVSFEDPF
310 320 330 340 350
AEDDWAAWSH FVSTTDIQIV GDDLTVTNPV RIKRAIEEKS ANALLLKVNQ
360 370 380 390 400
IGTITESIQA ANDSYDAKWG VMVSHRSGET EDVTIADLAV GLRAGQIKTG
410 420 430
APSRSERLAK YNQLLRIEEE LGDKAIFAGP KFHLSRAI
Length:438
Mass (Da):47,306
Last modified:August 16, 2004 - v1
Checksum:i278F4B3E25AEE03E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382132 Genomic DNA. Translation: CAG78318.1.
RefSeqiXP_505509.1. XM_505509.1.

Genome annotation databases

EnsemblFungiiCAG78318; CAG78318; YALI0_F16819g.
GeneIDi2907992.
KEGGiyli:YALI0F16819g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382132 Genomic DNA. Translation: CAG78318.1.
RefSeqiXP_505509.1. XM_505509.1.

3D structure databases

ProteinModelPortaliQ6C1F3.
SMRiQ6C1F3.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6C1F3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG78318; CAG78318; YALI0_F16819g.
GeneIDi2907992.
KEGGiyli:YALI0F16819g.

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiQ6C1F3.
KOiK01689.
OMAiSKWGGKG.
OrthoDBiEOG092C2W5X.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_YARLI
AccessioniPrimary (citable) accession number: Q6C1F3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: August 16, 2004
Last modified: April 12, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.