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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

DUT1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.By similarity

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871Substrate; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei132 – 1321SubstrateBy similarity

GO - Molecular functioni

  1. dITP diphosphatase activity Source: EnsemblFungi
  2. dUTP diphosphatase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dITP catabolic process Source: EnsemblFungi
  2. dUMP biosynthetic process Source: UniProtKB-UniPathway
  3. dUTP catabolic process Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:DUT1
Ordered Locus Names:YALI0F19448g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
ProteomesiUP000001300 Componenti: Chromosome F

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: EnsemblFungi
  2. nucleus Source: EnsemblFungi
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000182936Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi4952.Q6C141.

Structurei

3D structure databases

ProteinModelPortaliQ6C141.
SMRiQ6C141. Positions 3-119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 643Substrate bindingBy similarity
Regioni76 – 794Substrate bindingBy similarity
Regioni137 – 1382Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiCOG0756.
HOGENOMiHOG000028966.
InParanoidiQ6C141.
KOiK01520.
OMAiPKNTEAQ.
OrthoDBiEOG7T7H6C.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6C141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLKVAFLNE NARAPTRGSV HAAGYDLYAA EEKTIPANGR GLVDLGLSMS
60 70 80 90 100
IPEGTYARIA PRSGLAVKNG LSTGAGVIDY DYRGPVKVML FNHSTEDFNV
110 120 130 140 150
TIGDRVAQMI LERIVTPEVQ VVQTNDLEST ERGAGGFGST GINDEKKRKL

DEAEAKE
Length:157
Mass (Da):16,857
Last modified:August 15, 2004 - v1
Checksum:i1BB1C6A534555AEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382132 Genomic DNA. Translation: CAG78430.1.
RefSeqiXP_505621.1. XM_505621.1.

Genome annotation databases

EnsemblFungiiCAG78430; CAG78430; YALI0_F19448g.
GeneIDi2908309.
KEGGiyli:YALI0F19448g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382132 Genomic DNA. Translation: CAG78430.1.
RefSeqiXP_505621.1. XM_505621.1.

3D structure databases

ProteinModelPortaliQ6C141.
SMRiQ6C141. Positions 3-119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4952.Q6C141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG78430; CAG78430; YALI0_F19448g.
GeneIDi2908309.
KEGGiyli:YALI0F19448g.

Phylogenomic databases

eggNOGiCOG0756.
HOGENOMiHOG000028966.
InParanoidiQ6C141.
KOiK01520.
OMAiPKNTEAQ.
OrthoDBiEOG7T7H6C.

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDUT_YARLI
AccessioniPrimary (citable) accession number: Q6C141
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2004
Last sequence update: August 15, 2004
Last modified: March 31, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.