Q6C044 (ATG18_YARLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autophagy-related protein 18

Gene names

Name:	ATG18
Ordered Locus Names:	YALI0F27907g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia › Yarrowia lipolytica

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Necessary for proper vacuole morphology. Plays an important role in osmotically-induced vacuole fragmentation. Required for cytoplasm to vacuole transport (Cvt) vesicle formation, pexophagy and starvation-induced autophagy. Involved in correct ATG9 trafficking to the pre-autophagosomal structure. Might also be involved in premeiotic DNA replication By similarity.
Subunit structure	Component of the PI(3,5)P2 regulatory complex By similarity.
Subcellular location	Preautophagosomal structure membrane; Peripheral membrane protein By similarity. Vacuole membrane; Peripheral membrane protein By similarity. Endosome membrane; Peripheral membrane protein By similarity.
Domain	The N-terminus might form a beta-propeller domain involved in specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2), leading to the association of the protein to the membrane By similarity. The FRRGT-motif is essential for the cytoplasm to vacuole transport (Cvt) pathway, for the recruitment of ATG8 and ATG16 to the PAS in nutrient-rich medium, and for its recruitment to and dissociation from the PAS under starvation conditions By similarity.
Sequence similarities	Belongs to the WD repeat SVP1 family. Contains 2 WD repeats.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Endosome Membrane Vacuole
Domain	Repeat WD repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	pre-autophagosomal structure membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Autophagy-related protein 18		PRO_0000050873
Regions
	Repeat	193 – 233	41	WD 1
	Repeat	238 – 277	40	WD 2
	Motif	234 – 238	5	FRRGT-motif By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6C044 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 1C14722C0F67BBC0	FASTA	400	43,565
	10 20 30 40 50 60 MSDSINFVSF NQDYSCVSVG TPQGYKIYNC DPFGKCFSKA DGGMGIVEML FCTSLIAVVG 70 80 90 100 110 120 MGDQPQNSPR RLKIVNTKRQ STICELTFPT AVLGVRLNRQ RLVVLLQDQI YIYDISNMKL 130 140 150 160 170 180 VHTIETSPNP GAVCALSASS SDNNNYLVYP FPAPSSTAFN PGENNINDSS PNRKGDVTIF 190 200 210 220 230 240 DCNSLQPVNV VEAHKTPLAC LSLNSDGTLL ATASDKGTII RVFSVPKAQK LYEFRRGTYP 250 260 270 280 290 300 AQIFSINFNL ASNLMAVSSA TETVHIFQLE AGVSSTPEVP QDTELAIPTR TPQQKGMASV 310 320 330 340 350 360 FRKSSRSLGK GLAGAVGSYL PQTFTGMWEP LRDFAFIKQT SLPGTRSVVS VTSTNPPQVL 370 380 390 400 VVTLEGYFYQ YTLDLEKGGE CDLIRQYSLL DNVEGSLYGP

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382132 Genomic DNA. Translation: CAG78780.1.
RefSeq	XP_505968.1. XM_505968.1.
3D structure databases
ProteinModelPortal	Q6C044.
ModBase	Search...
Protein-protein interaction databases
STRING	4952.Q6C044.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2908106.
KEGG	yli:YALI0F27907g.
Phylogenomic databases
eggNOG	NOG317564.
HOGENOM	HOG000217543.
OMA	RGGDCIL.
OrthoDB	EOG4M0J97.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 1 hit. [Graphical view]
SMART	SM00320. WD40. 2 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG18_YARLI

Accession

Primary (citable) accession number: Q6C044

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2004
Last modified:	May 29, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program