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Protein

Endoplasmic reticulum chaperone BiP

Gene

KAR2

Organism
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.By similarity

Enzyme regulationi

The chaperone activity is regulated by ATP-induced allosteric coupling of the nucleotide-binding (NBD) and substrate-binding (SBD) domains. In the ADP-bound and nucleotide-free (apo) states, the two domains have little interaction. In contrast, in the ATP-bound state the two domains are tightly coupled, which results in drastically accelerated kinetics in both binding and release of polypeptide substrates. J domain-containing co-chaperones stimulate the ATPase activity and are required for efficient substrate recognition by HSPA5/BiP.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi57 – 60ATPBy similarity4
Nucleotide bindingi246 – 248ATPBy similarity3
Nucleotide bindingi312 – 319ATPBy similarity8
Nucleotide bindingi383 – 386ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum chaperone BiPCurated
Alternative name(s):
Immunoglobulin heavy chain-binding protein homologCurated
Short name:
BiPCurated
Gene namesi
Name:KAR2
Ordered Locus Names:DEHA2A01364g
OrganismiDebaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Taxonomic identifieri284592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeDebaryomyces
Proteomesi
  • UP000000599 Componenti: Chromosome A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Sequence analysisAdd BLAST34
ChainiPRO_000001358135 – 683Endoplasmic reticulum chaperone BiPAdd BLAST649

Proteomic databases

PRIDEiQ6BZH1

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ6BZH1
SMRiQ6BZH1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 299Nucleotide-binding (NBD)By similarityAdd BLAST155
Regioni419 – 519Substrate-binding (SBD)By similarityAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi680 – 683Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000228135
InParanoidiQ6BZH1
KOiK09490
OMAiCVGVMQK
OrthoDBiEOG092C1VPN

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6BZH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKVNSRFWI SGFTLAYVLL AVILPLLTPN QGQALAAEGE TDNDTESYGT
60 70 80 90 100
VIGIDLGTTY SCVGVMKNGK VEILANDQGN RITPSYVAFT PEERLIGDAA
110 120 130 140 150
KNQASSNVEN TVFDIKRLIG LKYNDKMVQK EIKHLPYKID KKDGKPVVKV
160 170 180 190 200
EFNDETKTFS PEEISGMILT KMKSIAEEYM GKKITHAVVT VPAYFNDAQR
210 220 230 240 250
QATKDAGTIA GLNVLRIVNE PTAAAIAYGL DKTEGEKQII VYDLGGGTFD
260 270 280 290 300
VSLLSIEGGV FEVLATAGDT HLGGEDFDFK VVRYLSSVFK KKHNIDISGN
310 320 330 340 350
SKAISKLKRE TEKAKRTLSS QMSTRIEIDS FVDGIDFSET LSRAKFEELN
360 370 380 390 400
IESFKKTLKP VQQVLKDAGF KKSDVDDIVL VGGSTRIPKV QELLEKFFDG
410 420 430 440 450
KKASKGINPD EAVAYGAAVQ AGVLSGEEGV HDIVLLDVNP LTLGIETTGG
460 470 480 490 500
VMTTLINRNT AIPTKKSQIF STAADNQPTV LIQVFEGERA LAKDNNKLGK
510 520 530 540 550
FELTSIPPAP RGVPQIEVTF SLDANGILKV EAMDKGTGKS ESITITNDKG
560 570 580 590 600
RLSKEDIDRM VEEAEKYAEQ DSELKAKIES RNSLENYAHM LKGQVKDESE
610 620 630 640 650
NGLGSKLNDD DKETLDDAIK ETLEFIEDNY DSATSEEFEE QKQKLIDVAS
660 670 680
PITSKLYGGG AGGADEAQFG DDDSDDEFVH DEL
Length:683
Mass (Da):74,585
Last modified:August 16, 2004 - v1
Checksum:i22CDC10E9EF3E479
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382133 Genomic DNA Translation: CAG84345.1
RefSeqiXP_456398.1, XM_456398.1

Genome annotation databases

EnsemblFungiiCAG84345; CAG84345; DEHA2A01364g
GeneIDi2899287
KEGGidha:DEHA2A01364g

Similar proteinsi

Entry informationi

Entry nameiBIP_DEBHA
AccessioniPrimary (citable) accession number: Q6BZH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: August 16, 2004
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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