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Q6BX78 (TRMB_DEBHA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
Ordered Locus Names:DEHA2B05258g
OrganismDebaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii) [Complete proteome]
Taxonomic identifier284592 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeDebaryomyces

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370595

Regions

Region129 – 1302S-adenosyl-L-methionine binding By similarity
Region166 – 1672S-adenosyl-L-methionine binding By similarity
Region264 – 2663S-adenosyl-L-methionine binding By similarity

Sites

Active site1891 By similarity
Binding site1061S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1861S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6BX78 [UniParc].

Last modified November 4, 2008. Version 2.
Checksum: 013E99298FE4683C

FASTA29234,725
        10         20         30         40         50         60 
MGKIEATSKE EKLRVQKEAE ARRRAYRDLK KEARQMQKEV KFDTDDNSEL PKKRFYRQRA 

        70         80         90        100        110        120 
HSNPFSDHRL EYPRSPDTMD WSKFYPQYYD SESKKMTSEV EIADIGCGYG GLLINLAPEF 

       130        140        150        160        170        180 
PDKLLLGMEI RVQVTQYVED RIIALRNQYR NELQNNYQNI SVLRGNAMKF LPNFFHKAQL 

       190        200        210        220        230        240 
SKMFFCFPDP HFKQRKHKAR IITNTLLAEY AYVLKEGGII YTITDVEDLH NWMVKHLEEH 

       250        260        270        280        290 
PMFERLSKEW EKQDKCVSIM WNSTEEGKKV TRNKGSKWVA CFKRLPTPDD CE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR382134 Genomic DNA. Translation: CAG85186.2.
RefSeqXP_457191.2. XM_457191.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4959.Q6BX78.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2913004.
KEGGdha:DEHA2B05258g.

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMARAHSNPI.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_DEBHA
AccessionPrimary (citable) accession number: Q6BX78
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 4, 2008
Last modified: June 11, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways