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Protein

5-aminolevulinate synthase, mitochondrial

Gene

HEM1

Organism
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of 5-aminolevulinate (ALA) from succinyl-CoA and glycine, the first and rate-limiting step in heme biosynthesis.By similarity

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.By similarity

Cofactori

pyridoxal 5'-phosphateBy similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase, mitochondrial (HEM1)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei124SubstrateBy similarity1
Binding sitei237SubstrateBy similarity1
Binding sitei256SubstrateBy similarity1
Binding sitei289Pyridoxal phosphateBy similarity1
Binding sitei317Pyridoxal phosphateBy similarity1
Binding sitei361Pyridoxal phosphateBy similarity1
Active sitei364By similarity1
Binding sitei393Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei394Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei479SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, mitochondrial (EC:2.3.1.37)
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene namesi
Name:HEM1
Ordered Locus Names:DEHA2B05478g
OrganismiDebaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Taxonomic identifieri284592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeDebaryomyces
Proteomesi
  • UP000000599 Componenti: Chromosome B

Subcellular locationi

  • Mitochondrion matrix By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55MitochondrionSequence analysisAdd BLAST55
ChainiPRO_000000123956 – 5755-aminolevulinate synthase, mitochondrialAdd BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei364N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6BX71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiQ6BX71.
KOiK00643.
OMAiKLAQYPK.
OrthoDBiEOG092C1OK6.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6BX71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESITRVSMS VCPFVKSSSA QALRQLSKNS ALTSQARQCP FMGAALNAKE
60 70 80 90 100
STRSYSSATK PVRATASSLA SNPPSTMQSK YSFKAEELVG NKDAINLESK
110 120 130 140 150
ENTFDFKGYL NSELSKKRTD KSYRFFNNIN RLANEFPKAH RSEENDKVTV
160 170 180 190 200
WCSNDYLGMG KNENTINEMK RVLTKYGSGA GGTRNIAGHN IHALKLESEL
210 220 230 240 250
AALHKHEAAL VFSSCFVAND AVLSLFGQKI KDLVIFSDEL NHASMIQGIR
260 270 280 290 300
NSRAKKQVFK HNDLADLEEK LAQYPKSTPK LIAFESVYSM CGSIAPIEAI
310 320 330 340 350
CDLAEKYGAL TFLDEVHAVG MYGPHGAGVA EHLDFDAHLK SGIASPQTQT
360 370 380 390 400
VMNRVDMVTG TLGKAYGTVG GYITGKANLI DWFRSFAPGF IFTTTLPPSI
410 420 430 440 450
MAGSSASIRY QRSTLQDRIA QQTNTRYVKN NLTDIGIPVI PNPSHIVPVL
460 470 480 490 500
VGNALDAKKA SDLLLDKYNI YVQAINFPTV PIGQERLRIT PTPGHGPELS
510 520 530 540 550
NQLIGALDSV FNELSLSRIG DWEGKGGLCG VGEPDIEPIE HIWTSEQLAL
560 570
TDADINPNVI DPVIQPIGVS SGVRD
Length:575
Mass (Da):62,652
Last modified:August 16, 2004 - v1
Checksum:i05107F73EB488A2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382134 Genomic DNA. Translation: CAG85193.1.
RefSeqiXP_457198.1. XM_457198.1.

Genome annotation databases

EnsemblFungiiCAG85193; CAG85193; DEHA2B05478g.
GeneIDi2913002.
KEGGidha:DEHA2B05478g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382134 Genomic DNA. Translation: CAG85193.1.
RefSeqiXP_457198.1. XM_457198.1.

3D structure databases

ProteinModelPortaliQ6BX71.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG85193; CAG85193; DEHA2B05478g.
GeneIDi2913002.
KEGGidha:DEHA2B05478g.

Phylogenomic databases

InParanoidiQ6BX71.
KOiK00643.
OMAiKLAQYPK.
OrthoDBiEOG092C1OK6.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM1_DEBHA
AccessioniPrimary (citable) accession number: Q6BX71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: August 16, 2004
Last modified: September 7, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.