Reviewed, UniProtKB/Swiss-Prot Q6BWG3 (TRM10_DEBHA)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    tRNA methyltransferase 10

Gene names

Name:	TRM10
Ordered Locus Names:	DEHA2B11572g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the RNA methyltransferase trmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	tRNA (guanine-N(1)-)-methyltransferase		PRO_0000060514

Sequences

	Sequence		Length	Mass (Da)
	Q6BWG3-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: AEB9E19616B39846	FASTA	360	41,793
	10 20 30 40 50 60 MENQDTEQSQ KRSGSEVEKD DFKRQKVVVP EGMTKREYKR QLKQQRWEET KDEYKQKKRE 70 80 90 100 110 120 KKKAARERRK ERIKEAEANG ETNEELYNYH QMKRAKVAPQ EQIDTDVKII MDCEFDSLMN 130 140 150 160 170 180 DKEIVSLSNQ ITRSYSAKKH STYDVQLDIT SFNKNLKKRF EKAIPQYDKW TNVTFVENDK 190 200 210 220 230 240 LEDILPMDDK QALSKYVYLT ADTDEVIDTL EPHHTYIIGG IVDKNRYKNL CLNKAQSLGL 250 260 270 280 290 300 KIGRLPIDKF IKMNGRQVLA TSHVFELCCK WFENDKDWGK AFNEVLPPRK VKGKLTHGSD 310 320 330 340 350 360 PEKSIEPSEV SEQPVSSEQS EQPVLSEQPV SSEQPVLSEQ PVLSESSDEP SDEPSKGADH

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382134 Genomic DNA. Translation: CAG85460.2. Different initiation.
RefSeq	XP_457456.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6BWG3.
Genome annotation databases
GeneID	2913391.
GenomeReviews	Gene locus DEHA2B11572g in contig CR382134_GR.
KEGG	dha:DEHA0B11814g.
Phylogenomic databases
eggNOG	fuNOG04600.
HOGENOM	HBG717479.
OrthoDB	EOG9NP8KZ.
Enzyme and pathway databases
BRENDA	2.1.1.31. 74267.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. [Graphical view]
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRM10_DEBHA

Accession

Primary (citable) accession number: Q6BWG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 16, 2008
Last modified:	January 19, 2010
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)