Q6BWA5 (IPYR_DEBHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	IPP1
Ordered Locus Names:	DEHA2B13090g

Organism

Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

284592 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Debaryomyces

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Inorganic pyrophosphatase		PRO_0000137582
Sites
	Metal binding	116	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 2 By similarity
	Metal binding	153	1	Magnesium 1 By similarity
	Binding site	79	1	Diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6BWA5 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 7A92B6D66F71F0B7	FASTA	287	32,140
	10 20 30 40 50 60 MSFTARQIGA PNTLDYRVFI EQNGKPVSPF HDIPLYANEE KTVLNMIVEV PRWTNAKLEI 70 80 90 100 110 120 SKEEKLNPIL QDTKKGKLRF VRNCFPHHGY IHNYGAFPQT WEDPNQVHPE TKAKGDNDPL 130 140 150 160 170 180 DVCEIGEQVG YVGQVKQVKV LGVMALLDEG ETDWKIIVID VNDPLASKLN DIEDVETHLP 190 200 210 220 230 240 GLLRATNEWF RIYKIPDGKP ENQFAFSGEC KNKKYAEEII NECSEAWEKL IKGETADSKG 250 260 270 280 ISLDNTTLSS TQTFSEAAAS GVPPASVQPA APIDKSVDKW FFISGAH

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382134 Genomic DNA. Translation: CAG85520.1.
RefSeq	XP_457514.1. XM_457514.1.
3D structure databases
ProteinModelPortal	Q6BWA5.
SMR	Q6BWA5. Positions 2-285.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6BWA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2913468.
GenomeReviews	Gene locus DEHA2B13090g in contig CR382134_GR.
KEGG	dha:DEHA2B13090g.
Phylogenomic databases
eggNOG	fuNOG04419.
HOGENOM	HBG434750.
OMA	WFFISGS.
OrthoDB	EOG4MGWHC.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_DEBHA

Accession

Primary (citable) accession number: Q6BWA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	August 16, 2004
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program