Reviewed, UniProtKB/Swiss-Prot Q6BV06 (BUR1_DEBHA)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase BUR1
    EC=2.7.11.22
    EC=2.7.11.23

Gene names

Name:	BUR1
Ordered Locus Names:	DEHA2C06358g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in transcription regulation. Phosphorylates the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B and the silencing of telomeric-associated genes. Also required for histone H3 methylation. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 608	608	Serine/threonine-protein kinase BUR1		PRO_0000085681
Regions
	Domain	39 – 346	308	Protein kinase
	Nucleotide binding	45 – 53	9	ATP By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Binding site	68	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6BV06-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: B27C11E4AAB8D95C	FASTA	608	69,467
	10 20 30 40 50 60 MECPEPSESV IPNDQSTKSC SIKLQPMNRF RDMSKLRNYE IIQKLGQGTF GVVQKARNIK 70 80 90 100 110 120 TKELVALKQL INHSAKEGFP ITAMREITIL KKLNHKNILK IIDMIYEEPK ISNPQDILHQ 130 140 150 160 170 180 RGCFYTVSPY MCSDLVGLLE NPNINLEVSH IKCFMEQLLH GIQYIHEQMF LHRDIKAANI 190 200 210 220 230 240 LIDRNGTLKI ADFGLARVYH GSPPKFMSGP GGGERAYTGL VVTRWYRPPE LLLGERRYTT 250 260 270 280 290 300 AVDMWGIGCV FGELFTRKPI LVGKTDSHQA QLIFDLVGPP NSISWSEATS LPNKHDLNIG 310 320 330 340 350 360 LTCQRSLESK FAPLMNPDGI NLLSGLLTLD PYKRFNALDA LNHNYFKNEP LPMKPQELPK 370 380 390 400 410 420 FEECHEIDKE RFKLLREKKN NIHEANKIPK AHFPKGPGEY NNSNNYPRNR NGSFPLALPK 430 440 450 460 470 480 QPKFYNQHQQ EAHVPQQMHT DTYIPKKRDD KPGANAPQKE SSEPITSYQS LRDRSPRREG 490 500 510 520 530 540 HISRKPSTTN SNNISSNSSA SNVGGTLSNP THQKNRPNAK ASAGIFMTNS RKQRPKPNPQ 550 560 570 580 590 600 SSSRNVSDQF KKRKLLPDEQ NESDLTDFDE DVKDSKQLDS FLDWDTFTRS PENRKLQHEK KQFETKYS

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382135 Genomic DNA. Translation: CAG86021.1.
RefSeq	XP_457963.1.
3D structure databases
SMR	Q6BV06. Positions 35-352.
ModBase	Search...
Genome annotation databases
GeneID	2900800.
GenomeReviews	Gene locus DEHA2C06358g in contig CR382135_GR.
KEGG	dha:DEHA0C07139g.
Phylogenomic databases
eggNOG	fuNOG04620.
OMA	GERRYTT.
OrthoDB	EOG96T4J6.
PhylomeDB	Q6BV06.
Enzyme and pathway databases
BRENDA	2.7.11.22. 74267. 2.7.11.23. 74267.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUR1_DEBHA

Accession

Primary (citable) accession number: Q6BV06

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)