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Protein

Cytosolic Fe-S cluster assembly factor NBP35

Gene

NBP35

Organism
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NBP35 and two labile, bridging clusters between subunits of the NBP35-CFD1 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi20 – 201Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi34 – 341Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi37 – 371Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi43 – 431Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi248 – 2481Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi251 – 2511Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 818ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NBP35UniRule annotation
Alternative name(s):
Nucleotide-binding protein 35UniRule annotation
Gene namesi
Name:NBP35UniRule annotation
Ordered Locus Names:DEHA2C14718g
OrganismiDebaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Taxonomic identifieri284592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeDebaryomyces
Proteomesi
  • UP000000599 Componenti: Chromosome C

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Cytosolic Fe-S cluster assembly factor NBP35PRO_0000278896Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of 2 NBP35 and 2 CFD1 chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6BTZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000079916.
InParanoidiQ6BTZ6.
OMAiIHVSGAG.
OrthoDBiEOG092C4BLN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6BTZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSQVEDIS KTELETPEHC PGPESEQAGK EDACNGCPNQ SICSSQLPQG
60 70 80 90 100
PDPDLPLINK RLSQIDHKIL VLSGKGGVGK STFTSMLSWA LAADEDIEVG
110 120 130 140 150
AMDLDICGPS LPRMLGAEGE SIHQSNSGWS PVYVADNLGL MSISFMLPDA
160 170 180 190 200
DSAVIWRGAK KNGLIKQFLK DVNWGEHLDY LVVDTPPGTS DEHLSVTTYM
210 220 230 240 250
KEVGIDGALI VTTPQEVALL DVRKEIDFCR KANIKILGLV ENMSGFVCPN
260 270 280 290 300
CKGESQIFRP TTGGGKKLCE DLKLPYLGAV PLDPRIGKAC DAGESFFDSY
310 320
ADSPASSAIL DVVDALRDQI EISLEKLNI
Length:329
Mass (Da):35,361
Last modified:August 16, 2004 - v1
Checksum:i3562A6ACB10B1FDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382135 Genomic DNA. Translation: CAG86403.1.
RefSeqiXP_458323.1. XM_458323.1.

Genome annotation databases

EnsemblFungiiCAG86403; CAG86403; DEHA2C14718g.
GeneIDi2900325.
KEGGidha:DEHA2C14718g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382135 Genomic DNA. Translation: CAG86403.1.
RefSeqiXP_458323.1. XM_458323.1.

3D structure databases

ProteinModelPortaliQ6BTZ6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG86403; CAG86403; DEHA2C14718g.
GeneIDi2900325.
KEGGidha:DEHA2C14718g.

Phylogenomic databases

HOGENOMiHOG000079916.
InParanoidiQ6BTZ6.
OMAiIHVSGAG.
OrthoDBiEOG092C4BLN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNBP35_DEBHA
AccessioniPrimary (citable) accession number: Q6BTZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: August 16, 2004
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.