Reviewed, UniProtKB/Swiss-Prot Q6BTC8 (DOT1_DEBHA)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specific
    EC=2.1.1.43
Alternative name(s):
    Histone H3-K79 methyltransferase
    H3-K79-HMTase

Gene names

Name:	DOT1
Ordered Locus Names:	DEHA2D01694g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	1172 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Enzyme regulation	Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 By similarity.
Subcellular location	Nucleus By similarity.
Miscellaneous	In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
Sequence similarities	Belongs to the DOT1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1172	1172	Histone-lysine N-methyltransferase, H3 lysine-79 specific		PRO_0000270611
Regions
	Region	1006 – 1008	3	S-adenosyl-L-methionine binding By similarity
	Region	1068 – 1069	2	S-adenosyl-L-methionine binding By similarity
	Motif	1002 – 1013	12	SAM-binding motif 1 By similarity
	Motif	1083 – 1092	10	SAM-binding motif 2 By similarity
Sites
	Binding site	984	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	1031	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6BTC8-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 29163B7AA796D97B	FASTA	1,172	135,295
	10 20 30 40 50 60 MENIAHHNTT YNITPICSDS SECSSMEMLP LGCKTHSDKS DARRNLMDTG IYTPDSNSDS 70 80 90 100 110 120 SDPRSDSSCV DVSNSLAVSH PISFCNWTDE ESSLLTEHIK TDLTSEALTS AFPNKSLALI 130 140 150 160 170 180 IRKIHDLQPV LDWTKNEIYL LAGIILNDSN SAIRRHKHKF PCRNVSNLNK KFQHYKNMVR 190 200 210 220 230 240 RLNGVDHSKW TKPEIASLIS LIDYDLTKTK LQKELPNKNI EEIKDLTNEM RIHSNFSHVE 250 260 270 280 290 300 SVLFEQTMTE NDPIEIVLDQ FPLKNKETCK KRLLKLNELS QHRDMAKRRL DEFESLIQNE 310 320 330 340 350 360 LKQIKDSIDL TRLKYLLVND LTGKQLRSSF PGISMKYLKL IAKEMGFDEA GEYTLAEMNF 370 380 390 400 410 420 LKKALQENAK LKSIIDELPF RSQLSIETKI NSVEPNRRRS VFTSQVDELL YMAKWYSSDN 430 440 450 460 470 480 FGNLSRRRNS RYASKLDKPK DKASDIMQPS KLHLENEEVT EAKVIPHDKG VENMISDHKL 490 500 510 520 530 540 KGKNNEQKKR KSKKPTSMVE VLKEESAYFQ SVTGNRCVLK EGQKRKRERL MQIKLETKLK 550 560 570 580 590 600 KPKSQNLNET NRIQETKKLM KCDVEPDKVK EQKSFKSENL KEAKLDASDS SIVSDVEISL 610 620 630 640 650 660 KKKLNKIENE EKRSPYDPED ISTDTLVPLY GRQLYVNEVY ETQPRPPKLS FREDTNIMVQ 670 680 690 700 710 720 NCSEISLTDT IAADIISQHC KNYRDMPISF PSLTIVDRNT NRMILNPMNK IRIRFLLYPQ 730 740 750 760 770 780 HSELFILAEP KSNELDPINE IKKLFQLHYS LFFSHSSKLK KIILSEYNKE IDISIEENDF 790 800 810 820 830 840 VRFMFVIDKW NRLMVELTPN DVDIGSHDIN EEIRAYLSPN EIKIPSDEDI RLDIFYSEIQ 850 860 870 880 890 900 LSTEENPISD NDPIEPSSPS FDLIKSMKRC FTHDSSNRLT PPISSEEDNK ENEPPIESDF 910 920 930 940 950 960 RNNNNKGSIP CTPVRLNTRN KMVVNAVKPE NYESNFFRHL KEKTSVSRFC VQQILLRIYS 970 980 990 1000 1010 1020 RIVSTESRKL RSYKAFTAEV YGELLPSFTS EVLTKVNLQP QHKFYDLGSG VGNTTFQAAL 1030 1040 1050 1060 1070 1080 EFGVHLSGGC EIMEHASKLT ELQTMLLNKH LALLGLKKLP LNFALSQSFV ENDIVRQAVI 1090 1100 1110 1120 1130 1140 ECDVLLVNNY LFDVNLNTAV GKMLYGLKPG TKIISLRNFI RPRYKASSDK TIFDYLKVER 1150 1160 1170 HEMSNYLSVS WTANKVPYYI STVQENICEE YV

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382136 Genomic DNA. Translation: CAG86674.2.
RefSeq	XP_458542.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2901204.
GenomeReviews	Gene locus DEHA2D01694g in contig CR382136_GR.
KEGG	dha:DEHA0D02387g.
Enzyme and pathway databases
BRENDA	2.1.1.43. 74267.
Family and domain databases
InterPro	IPR013110. DOT1. [Graphical view]
Pfam	PF08123. DOT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DOT1_DEBHA

Accession

Primary (citable) accession number: Q6BTC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	December 16, 2008
Last modified:	September 22, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)