Reviewed, UniProtKB/Swiss-Prot Q6BS98 (ALG1_DEBHA)

Names and origin

Protein names

Recommended name:
    Chitobiosyldiphosphodolichol beta-mannosyltransferase
    EC=2.4.1.142
Alternative name(s):
    GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
    GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
    Beta-1,4-mannosyltransferase
    Asparagine-linked glycosylation protein 1

Gene names

Name:	ALG1
Ordered Locus Names:	DEHA2D10516g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER By similarity.
Catalytic activity	GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitobiosyldiphosphodolichol beta-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Chitobiosyldiphosphodolichol beta-mannosyltransferase		PRO_0000080255
Regions
	Transmembrane	21 – 41	21	Potential
	Transmembrane	121 – 141	21	Potential
	Transmembrane	150 – 170	21	Potential
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Potential
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	217	1	N-linked (GlcNAc...) Potential
	Glycosylation	275	1	N-linked (GlcNAc...) Potential
	Glycosylation	421	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6BS98-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 2E96B40DF2F9E1F2	FASTA	472	54,464
	10 20 30 40 50 60 MEEFQFIKYK GFDHVFKYSG PWLWWLVGFY LCLPILAYTL LPYLTMNGTI SGKRKTVSIF 70 80 90 100 110 120 VLGDLGHSPR MCYHAKSFSK LDYYVNLCGY LEEQPPFDII DDINIDIYPI TVTKNTSNLP 130 140 150 160 170 180 FILFAAKKMV VQFFQLLKLL SDFRGTDYVL IQNPPSIPIL LIVLAYIKVF SRKTKLIIDW 190 200 210 220 230 240 HNLNYTILNL KFQNLKHPLV RILKTYERVL GQFADYNITV TRQMKEFLIK EFNFNKKKII 250 260 270 280 290 300 TLHDRPGEQF KPLESLGVTK QEILESHDIF RDIQNISKYK ILVSSTSFTP DEDFNLLLSA 310 320 330 340 350 360 LNQYDNSLAE RGLPPILIII TGKGPLKSQF LQKVKQLNFS DNVIIKNAWL SSEDYPLILS 370 380 390 400 410 420 VADLSISLHT SSSGIDLPMK IVDFFGCGIP VITLRFPAIG ELVTHGTNGL ITKSDKDSSV 430 440 450 460 470 NESQEIYRLL TEAFKNDELL DKIKQGALKE SNLRWEENWN NKMGKRFEYS TD

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382136 Genomic DNA. Translation: CAR65649.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6BS98.
Genome annotation databases
GenomeReviews	Gene locus DEHA2D10516g in contig CR382136_GR.
Phylogenomic databases
HOGENOM	Q6BS98.
OMA	TEISSEG.
Enzyme and pathway databases
BRENDA	2.4.1.142. 74267.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG1_DEBHA

Accession

Primary (citable) accession number: Q6BS98
Secondary accession number(s): B5RTG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 16, 2008
Last modified:	November 3, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)