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Protein

Isocitrate lyase

Gene

ICL1

Organism
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi172 – 1721MagnesiumBy similarity
Active sitei210 – 2101Proton acceptorBy similarity
Binding sitei247 – 2471SubstrateBy similarity
Binding sitei464 – 4641SubstrateBy similarity

GO - Molecular functioni

  1. isocitrate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:ICL1By similarity
Ordered Locus Names:DEHA2D12936g
OrganismiDebaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Taxonomic identifieri284592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeDebaryomyces
ProteomesiUP000000599 Componenti: Chromosome D

Subcellular locationi

Glyoxysome By similarity

GO - Cellular componenti

  1. peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 550550Isocitrate lyasePRO_0000068789Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi4959.Q6BRY4.

Structurei

3D structure databases

ProteinModelPortaliQ6BRY4.
SMRiQ6BRY4. Positions 8-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni101 – 1033Substrate bindingBy similarity
Regioni211 – 2122Substrate bindingBy similarity
Regioni430 – 4345Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2224.
InParanoidiQ6BRY4.
KOiK01637.
OMAiEMEQGVD.
OrthoDBiEOG73Z331.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6BRY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYTKIDVNE EEKFFQDQVK EIQQWWKEPR WAKTKRIYQA EDIAKKRSSL
60 70 80 90 100
KVDYPSSNQA KKLYKLLQEH DKNKSASFTF GALDPVQVTQ MAKYLDSIYV
110 120 130 140 150
SGWQCSSTAS TSNEPSPDLA DYPMDTVPNK VEHLWFAQLF HDRKQREERL
160 170 180 190 200
NLSKEERAKT PYTDFLRPII ADADTGHGGI TAILKLTKLF VERGAAGIHI
210 220 230 240 250
EDQAPGTKKC GHMAGKVLVP VQEHINRLVA IRASADILGS DLLCVARTDS
260 270 280 290 300
EAATLLTSTI DHRDHYFVLG ATNPESPDLA ALMAEAESNG IYGDKLASIE
310 320 330 340 350
VEWTKKAGLK LFHEAVIDEI NNGNFSNKQA LIKKFTDKVN PLSATSNKEA
360 370 380 390 400
RKLAREILGK DVFFDWDVAR AREGYYRYQG GTQCAVMRGQ AYAPYADMIW
410 420 430 440 450
MESALPDFKQ AKEFAEGVKA KWPDQWLAYN LSPSFNWNRA MPPNEQETYI
460 470 480 490 500
KRLSELGYVW QFITLAGLHT TALAVDDFAN QYSQIGMRAY GQTIQAPEIE
510 520 530 540 550
KGVEVVKHQK WSGAEYIDGL LKMVTGGVSS TAAMGAGVTE DQFKEKAAGK
Length:550
Mass (Da):61,776
Last modified:August 15, 2004 - v1
Checksum:iC570F0B76A9B47E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382136 Genomic DNA. Translation: CAG87204.1.
RefSeqiXP_459036.1. XM_459036.1.

Genome annotation databases

GeneIDi2901192.
KEGGidha:DEHA2D12936g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382136 Genomic DNA. Translation: CAG87204.1.
RefSeqiXP_459036.1. XM_459036.1.

3D structure databases

ProteinModelPortaliQ6BRY4.
SMRiQ6BRY4. Positions 8-527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4959.Q6BRY4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2901192.
KEGGidha:DEHA2D12936g.

Phylogenomic databases

eggNOGiCOG2224.
InParanoidiQ6BRY4.
KOiK01637.
OMAiEMEQGVD.
OrthoDBiEOG73Z331.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACEA_DEBHA
AccessioniPrimary (citable) accession number: Q6BRY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2004
Last sequence update: August 15, 2004
Last modified: March 31, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.