Q6BQ54 (MCR1_DEBHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 2
EC=1.6.2.2

Alternative name(s):
Mitochondrial cytochrome b reductase

Gene names

Name:	MCR1
Ordered Locus Names:	DEHA2E08228g

Organism

Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

284592 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Debaryomyces

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	NADH-cytochrome b5 reductase 2		PRO_0000330180
Regions
	Transmembrane	13 – 35	23	Helical; Potential
	Domain	50 – 154	105	FAD-binding FR-type
	Nucleotide binding	157 – 192	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6BQ54 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 048AD45D3CEFF00C	FASTA	299	33,153
	10 20 30 40 50 60 MSFTRQLTKN LTSFKVLAPF AAAVGSVGIA YQYSTSSIMN ETGKTFTGND EWIDLKLAKS 70 80 90 100 110 120 WDTTHNTKHF VFELANQDDV SGLINASCLL TKFVTPKGNN VIRPYTPISD VDGKGSIEFV 130 140 150 160 170 180 IKKYDGGKMS SHIHDLKPND TLAFKGPVVK WKWEPNQYKS IALIGGGTGI TPLYQLMHEI 190 200 210 220 230 240 TKNPEDKTKV NLFYGNLTEK DILIKKELDT IAEKHKDQVN VVYFLDKAPE NWNGETGYIS 250 260 270 280 290 KEFLQSKLPG PGKDNKVFVC GPPGLYKALS GPKKSPTDQG EVEGALADLG YTKENVFKF

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382137 Genomic DNA. Translation: CAG87900.1.
RefSeq	XP_459666.1. XM_459666.1.
3D structure databases
HSSP	HSSP built from PDB template 1UMK based on UniProtKB P00387.
ProteinModelPortal	Q6BQ54.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2901845.
GenomeReviews	Gene locus DEHA2E08228g in contig CR382137_GR.
KEGG	dha:DEHA2E08228g.
Phylogenomic databases
eggNOG	fuNOG07850.
HOGENOM	HBG591994.
OMA	IKRYENG.
OrthoDB	EOG4FFH9V.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
KO	K00326.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_DEBHA

Accession

Primary (citable) accession number: Q6BQ54

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 16, 2004
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program