Q6BNP0 (ERG27_DEBHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-keto-steroid reductase
EC=1.1.1.270

Gene names

Name:	ERG27
Ordered Locus Names:	DEHA2E20130g

Organism

Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

284592 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Debaryomyces

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols By similarity.
Catalytic activity	4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP+ = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-keto sterol reductase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	3-keto-steroid reductase		PRO_0000054592
Sites
	Active site	205	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6BNP0 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 15F6E90AA1EB0BB1	FASTA	346	39,154
	10 20 30 40 50 60 MIKDNDNSKV ALITGTSSNL GINIAYRLLE QLPSSTNVTL IVTSRTLPKV KEVITNINQY 70 80 90 100 110 120 SKTKLNRTGQ LEFDYLLVDF TDMVSVLSAY YDLNKKFKKI DYLFVNAAQG VYSGIDWVAA 130 140 150 160 170 180 TKEICRNPME GVTNPTYKTQ RVGVKSNDNM GLVFQANVFG PYYLIHKIKH LLQKGGRIIW 190 200 210 220 230 240 ISSLMSNPKY LSFNDLQLLK SPESYEGSKR LVDLMHFGTF KQLQSEYGIE QYLVQPGIFT 250 260 270 280 290 300 SFSFFKFLNV FTYYSMLMLF YLARLFGSPS HNISGFIAAN APVTCALGNE SQSVKVCSVS 310 320 330 340 NRTGKEYLSY QEVDTTGSAD ISAYLEKLCH EWDLTLKDQI VNTRQP

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382137 Genomic DNA. Translation: CAG88453.1.
RefSeq	XP_460180.1. XM_460180.1.
3D structure databases
ProteinModelPortal	Q6BNP0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6BNP0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2902497.
GenomeReviews	Gene locus DEHA2E20130g in contig CR382137_GR.
KEGG	dha:DEHA2E20130g.
Phylogenomic databases
eggNOG	fuNOG05767.
HOGENOM	HBG398311.
OMA	FLFYLAR.
OrthoDB	EOG4FBN2N.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K09827.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

ERG27_DEBHA

Accession

Primary (citable) accession number: Q6BNP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	August 16, 2004
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program