Reviewed, UniProtKB/Swiss-Prot Q6BNP0 (ERG27_DEBHA)

Names and origin

Protein names

Recommended name:
    3-keto-steroid reductase
    EC=1.1.1.270

Gene names

Name:	ERG27
Ordered Locus Names:	DEHA2E20130g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols By similarity.
Catalytic activity	4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP+ = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-keto-steroid reductase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	3-keto-steroid reductase		PRO_0000054592
Sites
	Active site	205	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6BNP0-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 15F6E90AA1EB0BB1	FASTA	346	39,154
	10 20 30 40 50 60 MIKDNDNSKV ALITGTSSNL GINIAYRLLE QLPSSTNVTL IVTSRTLPKV KEVITNINQY 70 80 90 100 110 120 SKTKLNRTGQ LEFDYLLVDF TDMVSVLSAY YDLNKKFKKI DYLFVNAAQG VYSGIDWVAA 130 140 150 160 170 180 TKEICRNPME GVTNPTYKTQ RVGVKSNDNM GLVFQANVFG PYYLIHKIKH LLQKGGRIIW 190 200 210 220 230 240 ISSLMSNPKY LSFNDLQLLK SPESYEGSKR LVDLMHFGTF KQLQSEYGIE QYLVQPGIFT 250 260 270 280 290 300 SFSFFKFLNV FTYYSMLMLF YLARLFGSPS HNISGFIAAN APVTCALGNE SQSVKVCSVS 310 320 330 340 NRTGKEYLSY QEVDTTGSAD ISAYLEKLCH EWDLTLKDQI VNTRQP

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382137 Genomic DNA. Translation: CAG88453.1.
RefSeq	XP_460180.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6BNP0.
Genome annotation databases
GeneID	2902497.
GenomeReviews	Gene locus DEHA2E20130g in contig CR382137_GR.
KEGG	dha:DEHA0E21263g.
Phylogenomic databases
HOGENOM	Q6BNP0.
OMA	EQYLVHP.
Enzyme and pathway databases
BRENDA	1.1.1.270. 74267.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

ERG27_DEBHA

Accession

Primary (citable) accession number: Q6BNP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)