Reviewed, UniProtKB/Swiss-Prot Q6BND2 (CET1_DEBHA)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme subunit beta
    EC=3.1.3.33
Alternative name(s):
    Polynucleotide 5'-triphosphatase
    mRNA 5'-triphosphatase
      Short name=TPase

Gene names

Name:	CET1
Ordered Locus Names:	DEHA0E24112g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end By similarity.
Catalytic activity	A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate.
Cofactor	Divalent ions By similarity.
Subunit structure	The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase By similarity.
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the fungal TPase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: InterPro
Molecular function	polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	mRNA-capping enzyme subunit beta		PRO_0000210115

Sequences

	Sequence		Length	Mass (Da)
	Q6BND2-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 562F512ECD5B9214	FASTA	476	54,700
	10 20 30 40 50 60 MNVGSILNDE PSPSKKTTND DSGDSVRPDM STYQRHSLVN LLNDPAPNNE LKTKETKKDW 70 80 90 100 110 120 SEDEHSNFRV PVTEPNQQLS PVLRRSSIAD ITNEKDVDIS SSTEHPIEQD KSEKDEDELT 130 140 150 160 170 180 RISKLKSTNK PRRYTEPPIW AQEWIPTSYQ GTANGTPVVN AQESSLSSKR VFDRSLTVNV 190 200 210 220 230 240 DLECSITGVI PPPSVTRTIA EWIYANFTEI PDDQRKYVEL ELKFGTIIDK RAGHRIDINV 250 260 270 280 290 300 STECIFTDNS NTYFDMGVHE VGWNDMCKFL DDLEKSYQDE LRRSPQANSN SPKRKFNILE 310 320 330 340 350 360 SDITDNFYQI TSRNEQPKSI RISKDNLLDP PRYTAINKQR LSSLFIHNPS SMYDLRLSLS 370 380 390 400 410 420 YENPIADNNI DGIIKKNQPT LTRIKKRNSW THRPTVTRFD MTRVLLPREL KNKSGKKIVE 430 440 450 460 470 QDQSFEVELE VDTLELFNGF DKFKSGADSI RFEELVEIFV NNARCLNNRV TKLANK

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382137 Genomic DNA. Translation: CAG88572.1.
RefSeq	XP_460288.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2903003.
KEGG	dha:DEHA0E24112g.
Phylogenomic databases
HOGENOM	Q6BND2.
Family and domain databases
InterPro	IPR004206. mRNA_triPase. [Graphical view]
Pfam	PF02940. mRNA_triPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CET1_DEBHA

Accession

Primary (citable) accession number: Q6BND2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)