Q6BN88 (ATG26_DEBHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sterol 3-beta-glucosyltransferase
EC=2.4.1.173

Alternative name(s):
Autophagy-related protein 26

Gene names

Name:	ATG26
Ordered Locus Names:	DEHA2E23738g

Organism

Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

284592 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Debaryomyces

Protein attributes

Sequence length	1574 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1574	1574	Sterol 3-beta-glucosyltransferase		PRO_0000215609
Regions
	Domain	253 – 288	36	GRAM 1
	Domain	323 – 471	149	PH
	Domain	854 – 920	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	Q6BN88 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 7435219832B0944C	FASTA	1,574	177,279
	10 20 30 40 50 60 MLPKEDKVNS NHFKGVNDAH KTSLYKRLSS SVIPPLTFLN QNPASPNNEE VPGNNEANKD 70 80 90 100 110 120 EKTFSDDEDI AHPIGSVGSS NNFLSFLYAG MGKLSDLKGN DASNANEAKD SKLNDNLRSS 130 140 150 160 170 180 RNEQANEPEY RREYKLDYDI DESEEDDIES TRDENTLKPK TEDTSVHSKL DPEERLENVV 190 200 210 220 230 240 NMLFDDHDES TTALASDPSK GTVFQQSVLK NFDPFRIQQT ELLKLKDITL STEEDESEEQ 250 260 270 280 290 300 KLKKKFFRLK VADKLKRVFE LNDDDYFYGN YNVWLVRDVL LQGHIYLTKE SILFFTFLPK 310 320 330 340 350 360 RHNTISASKG TTGGFQHHDD SHDVIQSGSL GMKTALYGDT VFSTPLTHRF WVILRNETIT 370 380 390 400 410 420 VYHSPTDLYF PITLIDLKSC VRAEVIEKGR NDNASPRPDL HRNDSQEVSS GDEEVEFSNM 430 440 450 460 470 480 LNSNYQLEDN SENVSGGYWF KVVTKKKTHK FHSDSLYSAR QWVNNIVKVV FQLHNSNANN 490 500 510 520 530 540 EVIMKIPIND VLSFDLNEVF GASEKNSDSN GGEEKPKVLN VKYLENGSKG RYALSASRMK 550 560 570 580 590 600 KELKNKTKKK MKKNSGNEPD ELLSENTYFL LFKDGDEVFS TLNEIVNENH HSSNIFRNRH 610 620 630 640 650 660 NSISKKYTSE KGSNWNRPFT HKDEEIHIPR AISTLTTDHF QNSIIDQIEE ASHRNTHTQN 670 680 690 700 710 720 TDLASPRSEC GLTHSVSTSP QSKIRKFGKT LITPSKIFSN KSRTESEKST PDRSQTTSPV 730 740 750 760 770 780 QGINLSLSGL KDLNMAFEAS QKNYEVSCTR YSHTEENSTN SPSNFQNNTL SKADALSPQI 790 800 810 820 830 840 KSPQPLEAGP LNLTDPSEYE DNKKKNSTLS SIGKSIKAMS SIRSKLAAVN HYEQLDENDT 850 860 870 880 890 900 YFIRDVSARE VDTRHFQERF SFNNKKQLIA SYHCHIIRAV PVFGKVYLGD SEICFRSMLP 910 920 930 940 950 960 GVSTRMILPL IDVDTCSKEK GSNIAYSGLV LVIRGYDELF MEFSVQSARD DCLAMILRQL 970 980 990 1000 1010 1020 EKNRESGNES SDDNKSAQHG KSGCFQKTPS SAETTKSSNE IKLAQWRIEN ARLKLFEDKI 1030 1040 1050 1060 1070 1080 NAAAGLHVPI VLEDSPFYKT EIRPSTSFNF TLLTIGSRGD VQPYIALGKG LLNEGHNVTI 1090 1100 1110 1120 1130 1140 ATHSDFEEWI VGHGIKFKTI AGNPVELMSL MVTHGSMSLS FLKEASSKFR GWIQELLDTS 1150 1160 1170 1180 1190 1200 WKACQGSDIL IESPSAMVGA HIAEALGIPY IRAFTMPWTR TRAYPHAFIV PDKKKGGSYN 1210 1220 1230 1240 1250 1260 YITHLMFETV LWKGISSQVN KWRRESLGLP RTNLYRLAQY DIPFLYNISP TIFPPSVDFP 1270 1280 1290 1300 1310 1320 DWVKVTGYWF LDEGAADDFE PSKELVEFMN KARADDKKVV YIGFGSIVVE DAKSLTKAIV 1330 1340 1350 1360 1370 1380 EAVLNADVRC ILNKGWSDRN SSPAKDNAEP EVELPEEIYN SGSIPHDWLF PKIDAAVHHG 1390 1400 1410 1420 1430 1440 GSGTTGATMR AGIPTIIKPF FGDQFFYSSR IEDIGAGIGL KKLNARSLCT ALKTATSDAK 1450 1460 1470 1480 1490 1500 MITKAKKISE RLKQENGVLN AIEAIYYELE YARSLILAKQ HENTKHDLKS GTQTPVVNET 1510 1520 1530 1540 1550 1560 NEYFDSDTYD ADHDSDKESD HDQTYEQDNH SDYDVANDDN MTEIVEPSLE DGNDTVRIAP 1570 DSGNDNTTVT DANK

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382137 Genomic DNA. Translation: CAR65878.1.
RefSeq	XP_002770537.1. XM_002770491.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6BN88.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8998839.
GenomeReviews	Gene locus DEHA2E23738g in contig CR382137_GR.
KEGG	dha:DEHA2E23738g.
Phylogenomic databases
eggNOG	fuNOG07241.
HOGENOM	HBG397878.
OMA	DVLLQGH.
OrthoDB	EOG48KVK8.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 2 hits.
KO	K05841.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 1 hit. PF00169. PH. 1 hit. PF00201. UDPGT. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_DEBHA

Accession

Primary (citable) accession number: Q6BN88
Secondary accession number(s): B5RU66

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	December 16, 2008
Last modified:	November 16, 2011
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program