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Q6BM98 (LYS4_DEBHA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homoaconitase, mitochondrial

EC=4.2.1.36
Alternative name(s):
Homoaconitate hydratase
Gene names
Name:LYS4
Ordered Locus Names:DEHA2F07238g
OrganismDebaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii) [Complete proteome]
Taxonomic identifier284592 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeDebaryomyces

Protein attributes

Sequence length688 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible hydration of cis-homoaconitate to (2R,3S)-homoisocitrate, a step in the alpha-aminoadipate pathway for lysine biosynthesis By similarity.

Catalytic activity

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O.

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 3/5.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandIron
Iron-sulfur
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysine biosynthetic process via aminoadipic acid

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: InterPro

homoaconitate hydratase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1919Mitochondrion Potential
Chain20 – 688669Homoaconitase, mitochondrial
PRO_0000247923

Sites

Metal binding3461Iron-sulfur (4Fe-4S) By similarity
Metal binding4061Iron-sulfur (4Fe-4S) By similarity
Metal binding4091Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6BM98 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: B1E0A2C936163F89

FASTA68874,358
        10         20         30         40         50         60 
MALLYLSTRS SLKKTGARCL SSSSILYRGQ NLTEKIVQKY AVGLADDKKV FSGDYVTIKP 

        70         80         90        100        110        120 
AHCMSHDNSW PVATKFMGLG ATNVHDNRQI VCTLDHDVQN KSEKNLEKYH NIENFAKEQG 

       130        140        150        160        170        180 
IDFYPAGRGI GHQIMIEEGY AYPLNLTVAS DSHSNTYGGI GSLGTPIVRT DAASIWATGQ 

       190        200        210        220        230        240 
TWWQIPPVAK VELVGQLPKG ATGKDIIVAL CGIFNNDEVL NHAIEFTGEG VKNLSVDYRL 

       250        260        270        280        290        300 
TIANMTTEWG ALSGLFPVDE TLVNFYDERV KKLPQPHPRV NENTVKNLSE NKVNSDNDAV 

       310        320        330        340        350        360 
YAKHLVVDLS SLSPYISGPN SVKVSTSLHD LSKQNIKINK AYLVSCTNSR LSDIKAAADI 

       370        380        390        400        410        420 
IKGHKVAEGV EFYVAAASSN VQKDAEAIGA WQDIIDAGAK PLPAGCGPCI GLGTGLLEDG 

       430        440        450        460        470        480 
EVGISATNRN FKGRMGSKDA LAYLASPEVV AASAVLGKIG GPEELQGNPV PTNPEIVRSI 

       490        500        510        520        530        540 
TSNESTETVD PDASGSSVDV LDGFPQSIEG ELILCDADNI NTDGIYPGKY TYQDDIPRSK 

       550        560        570        580        590        600 
MAEVCMENYD SEFKQKTKAG DIIISGYNFG TGSSREQAAT AILARDMKLV VAGSFGNIFS 

       610        620        630        640        650        660 
RNSINNALLT LEIPALINKL REKYAQSNEL TIRTGWFLKW DVTKNQVTVS DSEGNLILTQ 

       670        680 
KVGELGTNLQ DIIVKGGLEG WVKAQLKN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR382138 Genomic DNA. Translation: CAG89007.1.
RefSeqXP_460673.1. XM_460673.1.

3D structure databases

ProteinModelPortalQ6BM98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4959.Q6BM98.

Proteomic databases

PRIDEQ6BM98.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2903254.
KEGGdha:DEHA2F07238g.

Phylogenomic databases

eggNOGCOG1048.
HOGENOMHOG000173778.
KOK01705.
OMASWPVATK.
OrthoDBEOG70CRGK.

Enzyme and pathway databases

UniPathwayUPA00033; UER01027.

Family and domain databases

Gene3D3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
IPR004418. Homoaconitase_mito.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PTHR11670:SF8. PTHR11670:SF8. 1 hit.
PfamPF00330. Aconitase. 2 hits.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsTIGR00139. h_aconitase. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLYS4_DEBHA
AccessionPrimary (citable) accession number: Q6BM98
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: August 16, 2004
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways