Reviewed, UniProtKB/Swiss-Prot Q6BLM0 (ATG15_DEBHA)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	ATG15
Ordered Locus Names:	DEHA2F12364g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 615	615	Putative lipase ATG15		PRO_0000090367
Regions
	Topological domain	1 – 22	22	Cytoplasmic Potential
	Transmembrane	23 – 43	21	Signal-anchor for type II membrane protein Potential
	Topological domain	44 – 615	572	Lumenal Potential
	Compositional bias	537 – 585	49	Ser-rich
Sites
	Active site	378	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	253	1	N-linked (GlcNAc...) Potential
	Glycosylation	276	1	N-linked (GlcNAc...) Potential
	Glycosylation	360	1	N-linked (GlcNAc...) Potential
	Glycosylation	551	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6BLM0-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 81F12D95ECBF55D9	FASTA	615	69,083
	10 20 30 40 50 60 MKQLGEEHPL ISTKRPRAKK RRSIAICAAV LTLIAFGFIR FVPKDILAGG WYEGEKDGLS 70 80 90 100 110 120 ALDNEEVRDM QTDGTTFELK HIFHRGTGSH NYMVHRRLDV TKEYLEAHSE ELEELTTMQT 130 140 150 160 170 180 SEVDESNLQD VYDAYDWPQA HTGKNPWTIK LQIRQSPTNG VVKRLKERHT PNFLDSYLAY 190 200 210 220 230 240 ALSVKGNPAL LNKIELEWED EHEIPIPDVK DRDTVVSLAT ISSNAYVKFP KDDKEKKRSD 250 260 270 280 290 300 WIDVGDPWVP DENHTDINFG WADDGLRGHV FVSTDNKTVV IGIKGTSGAG LIGTGPEETT 310 320 330 340 350 360 ANDKLNDNLL FSCCCARVGY MWTTVCDCYQ KAYTCDQDCL EKELMRQDRY YQATLDLYRN 370 380 390 400 410 420 VSQLYDPSTT NIWVTGHSLG GALASLVGRT YGLPAVAFEA PGEMLATRRL HLPQPPGLPK 430 440 450 460 470 480 HLENIWHFGN TADPIYMGVC NGVSSSCNVA GYAMETACHT AHQCVYDVVT DMGWRVNLLN 490 500 510 520 530 540 HRIHTVIDDI ILAYNDTPPC VQQPPCRDCF NWRFTSSDDN KNDEPPLPNP LHPKPPSTVR 550 560 570 580 590 600 SSNMPHEQSP NASRSLSSLC TEPSCTSSFQ SVSPSFSSQL PSHPSQNPQR CLRRTWYGRC 610 SKWGHNSAAH HVSSI

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382138 Genomic DNA. Translation: CAR66329.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6BLM0.
Genome annotation databases
GenomeReviews	Gene locus DEHA2F12364g in contig CR382138_GR.
Phylogenomic databases
HOGENOM	Q6BLM0.
OMA	DINIPNI.
Enzyme and pathway databases
BRENDA	3.1.1.3. 74267.
Family and domain databases
InterPro	IPR008262. Lipase_Ser_AS. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_DEBHA

Accession

Primary (citable) accession number: Q6BLM0
Secondary accession number(s): B5RUF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	December 16, 2008
Last modified:	November 3, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)