Reviewed, UniProtKB/Swiss-Prot Q6BKG0 (PPN1_DEBHA)

Names and origin

Protein names

Recommended name:
    Endopolyphosphatase
    EC=3.6.1.10

Gene names

Name:	PPN1
Ordered Locus Names:	DEHA2F22264g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of inorganic polyphosphate (poly P) chains of many hundreds of phosphate residues into shorter lengths By similarity.
Catalytic activity	Polyphosphate + n H2O = (n+1) oligophosphate.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein.
Post-translational modification	Processing by proteases in the vacuole may be required for activation By similarity.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endopolyphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Endopolyphosphatase		PRO_0000058546
Regions
	Topological domain	1 – 19	19	Cytoplasmic Potential
	Transmembrane	20 – 40	21	Signal-anchor for type II membrane protein Potential
	Topological domain	41 – 713	673	Vacuolar Potential
	Compositional bias	395 – 415	21	Asp-rich
	Compositional bias	638 – 660	23	Lys-rich
Amino acid modifications
	Glycosylation	507	1	N-linked (GlcNAc...) Potential
	Glycosylation	645	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6BKG0-1 [UniParc]. Last modified December 16, 2008. Version 3. Checksum: 1DD7BA09E78B4897	FASTA	713	81,885
	10 20 30 40 50 60 MSVLIDEKSH RSSGSTRSRI VVTVVGVLLM VSGLAVMLGH QSGSANEALG MEYEKPSVEV 70 80 90 100 110 120 LDLEATEVEQ LTKLGLSPKP KLKIVKGGDE QVLHGRFLHI TDMHPDKYYK TGADVGSLCH 130 140 150 160 170 180 SGKGSAGKYG DAVLGCDSPM VLMEDTLKWV KENLKDKIDF VVWTGDNVRH DNDRRYPRTE 190 200 210 220 230 240 SNIFDMNQRV SELMYETFKE ENPRGGRPRQ LKIPLVPSLG NNDVFPHNLF SPGPTLQTRE 250 260 270 280 290 300 LFKIWHDFVP AAQLHIFNRG AYFFKEIIPN ELAVLSINTL YLFQSNPLVD NCDRKKDPGH 310 320 330 340 350 360 KLFEWLGYTL KEMRARNMKV WLSGHVPPNE KNYDISCLRK YIVWMHEYRD VIIGGLYGHM 370 380 390 400 410 420 NIDHFIPLDS KEAYKSIKNK FGKLGFDYEL SFENDLYVSD DDDNSDSDSD DDDEDTSLEE 430 440 450 460 470 480 SYSNFNSPIL KDGFEDSVNF KNMDDIRIQG GVPNGKVGYM ENVRKEYYAN VKGKKKSGYV 490 500 510 520 530 540 SERYSIAHVT ASVVPTFNSG LRVWEYNITG LQNLLTSDNQ PRFAPWNEFF EGLEKLMETQ 550 560 570 580 590 600 VEADYDDEFI TFGQQVEIFK NDNTFPPKMP KSKSLGPAYI PQAFTPERYV QYYADLANIN 610 620 630 640 650 660 RGEKEFSYEF EYATDDKVYD MDSLTVDDWI SLARRLGKPV KEKKNKSNKK SKKKKKNKDK 670 680 690 700 710 RLLENSEPLK QDGSKDSRLE QDRVQQSARL ENLWQHYLKY SFVSSEYENM GMG

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382138 Genomic DNA. Translation: CAG89712.2.
RefSeq	XP_461311.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2904257.
KEGG	dha:DEHA0F23661g.
Phylogenomic databases
HOGENOM	Q6BKG0.
OMA	Q6BKG0. GGLYGHM.
Enzyme and pathway databases
BRENDA	3.6.1.10. 74267.
Family and domain databases
InterPro	IPR012358. EndopolyPtase_N1. IPR004843. M-pesterase. [Graphical view]
PANTHER	PTHR10340:SF4. EndopolyPtase_N1. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPN1_DEBHA

Accession

Primary (citable) accession number: Q6BKG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	December 16, 2008
Last modified:	June 16, 2009
This is version 31 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)