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Protein

Enolase 2

Gene

ENO2

Organism
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GPD)
  2. no protein annotated in this organism
  3. Phosphoglycerate mutase (DEHA2E21054g), Phosphoglycerate mutase (DEHA2D12760g)
  4. Enolase 1 (ENO1), Enolase 2 (ENO2)
  5. Pyruvate kinase (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160SubstrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei212Proton donorBy similarity1
Metal bindingi247MagnesiumBy similarity1
Metal bindingi296MagnesiumBy similarity1
Binding sitei296SubstrateBy similarity1
Metal bindingi323MagnesiumBy similarity1
Binding sitei323SubstrateBy similarity1
Active sitei348Proton acceptorBy similarity1
Binding sitei399SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 2 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 2
2-phosphoglycerate dehydratase 2
Gene namesi
Name:ENO2
Ordered Locus Names:DEHA2G14058g
OrganismiDebaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Taxonomic identifieri284592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeDebaryomyces
Proteomesi
  • UP000000599 Componenti: Chromosome G

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340511 – 439Enolase 2Add BLAST439

Proteomic databases

PRIDEiQ6BI20.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6BI20.
SMRiQ6BI20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni375 – 378Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiQ6BI20.
KOiK01689.
OMAiDSFDATW.
OrthoDBiEOG092C2W5X.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6BI20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVTKLFARY VYDSRGNPTV EVDLTTDKGL FRSIVPSGAS TGIHEALELR
60 70 80 90 100
DGDKSKWLGK GVTKAVANVN ELIAPAFVKA NLDVTNQSAV DDFLNKLDGT
110 120 130 140 150
PNKSKLGANA ILGVSLATAK AAAAEKNVPL YQHLADISGS KQDKFVLPVP
160 170 180 190 200
FQNVLNGGSH AGGALAFQEF MIVPTDAPTF SEALRMGSEV YHNLKSLTKK
210 220 230 240 250
KYGQSAGNVG DEGGVAPDIG SPREALDLIM DAIDKAGYKG KIGIALDVAA
260 270 280 290 300
SEFYKDGKYD LDFKNPNSDE SKWLSGEQLA SLYEELINEY PIVSIEDPFG
310 320 330 340 350
EDDWDAWVHF YSKVSSKVQI VGDDLTVTNP LRIKTAIEKK AANALLLKVN
360 370 380 390 400
QIGSLTESIK AAQDSFDATW GVMVSHRSGE TEDTFIADLS VGLRAGQIKT
410 420 430
GAPARSERLA KLNQILRIEE ELGDKAIYAG KNFHAASQL
Length:439
Mass (Da):47,239
Last modified:August 16, 2004 - v1
Checksum:i434D235716664DB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382139 Genomic DNA. Translation: CAG90637.1.
RefSeqiXP_462151.1. XM_462151.1.

Genome annotation databases

EnsemblFungiiCAG90637; CAG90637; DEHA2G14058g.
GeneIDi2905065.
KEGGidha:DEHA2G14058g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382139 Genomic DNA. Translation: CAG90637.1.
RefSeqiXP_462151.1. XM_462151.1.

3D structure databases

ProteinModelPortaliQ6BI20.
SMRiQ6BI20.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6BI20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG90637; CAG90637; DEHA2G14058g.
GeneIDi2905065.
KEGGidha:DEHA2G14058g.

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiQ6BI20.
KOiK01689.
OMAiDSFDATW.
OrthoDBiEOG092C2W5X.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO2_DEBHA
AccessioniPrimary (citable) accession number: Q6BI20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: August 16, 2004
Last modified: March 15, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.