Reviewed, UniProtKB/Swiss-Prot Q6BI19 (KYNU_DEBHA)

Names and origin

Protein names

Recommended name:
    Kynureninase
    EC=3.7.1.3
Alternative name(s):
    L-kynurenine hydrolase
    Biosynthesis of nicotinic acid protein 5

Gene names

Name:	BNA5
Ordered Locus Names:	DEHA2G14080g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively By similarity.
Catalytic activity	L-kynurenine + H2O = anthranilate + L-alanine. L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1. Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kynureninase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro tryptophan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kynureninase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Kynureninase		PRO_0000218662
Regions
	Region	144 – 147	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	116	1	Pyridoxal phosphate; via amide nitrogen By similarity
	Binding site	117	1	Pyridoxal phosphate By similarity
	Binding site	228	1	Pyridoxal phosphate By similarity
	Binding site	231	1	Pyridoxal phosphate By similarity
	Binding site	253	1	Pyridoxal phosphate By similarity
	Binding site	288	1	Pyridoxal phosphate By similarity
	Binding site	316	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	254	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6BI19-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 56DB4A0D03FBFF9C	FASTA	460	52,093
	10 20 30 40 50 60 MSIEQAKKLD ECFPTYKEEF EIPTFKSLGI QNDEYEDSTD SIYLCGNSLG LMPKITRTAI 70 80 90 100 110 120 NDELNAWSER GVESHFRHPG EEKGLTSWVD IDLPLLPLIA PIVGGKENEV AVMGTLTSNL 130 140 150 160 170 180 NAMLMSFYKP SGKKTKILFE KQAFPSDYYA FLNAAKIFGY NEDHLIQIEI KEGKTYIETE 190 200 210 220 230 240 DIIETITNHQ DELALVCFSG IQYYTGQFFN IGEITECAKS FGITVGWDLA HAVGNVPLQL 250 260 270 280 290 300 HDWDVDFAVW CSYKYLNSGP GGIAGIFVHE KHTKDNSIEQ FKPRLAGWWG NNASDRFKML 310 320 330 340 350 360 EVFDPIKSAL SYRQSNPSVI DVVAVKSSLE LFKKVGGISE LRKKSVELTG FLQALLTSSK 370 380 390 400 410 420 YYIKQEETTD RLGFKILSPL NKGDRGCQLS VLFQPHYEEY SKNIMERVNK YLSDHAIVCD 430 440 450 460 ERRPDVIRLA PLPLYNTFSE TFIAVQRLIE AMDKIAANEI

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382139 Genomic DNA. Translation: CAG90638.1.
RefSeq	XP_462152.1.
3D structure databases
SMR	Q6BI19. Positions 5-453.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6BI19.
Genome annotation databases
GeneID	2905066.
GenomeReviews	Gene locus DEHA2G14080g in contig CR382139_GR.
KEGG	dha:DEHA0G14949g.
Phylogenomic databases
eggNOG	fuNOG06509.
HOGENOM	HBG523016.
OMA	KPRLAGW.
OrthoDB	EOG9RZ0G9.
PhylomeDB	Q6BI19.
Enzyme and pathway databases
BRENDA	3.7.1.3. 74267.
Family and domain databases
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR010111. Kynureninase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR14084. Kynureninase. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
TIGRFAMs	TIGR01814. kynureninase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KYNU_DEBHA

Accession

Primary (citable) accession number: Q6BI19

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)