Q6BHA8 (BUD32_DEBHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase BUD32
EC=2.7.11.1

Gene names

Name:	BUD32
Ordered Locus Names:	DEHA2G19998g

Organism

Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

284592 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Debaryomyces

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation By similarity. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Component of the EKC/KEOPS complex By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Chromosome › telomere By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosome Cytoplasm Nucleus Telomere
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Serine/threonine-protein kinase BUD32		PRO_0000278913
Regions
	Domain	15 – 260	246	Protein kinase
	Nucleotide binding	21 – 29	9	ATP By similarity
Sites
	Active site	162	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6BHA8 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: EF45BE76F6FDDD03	FASTA	260	29,991
	10 20 30 40 50 60 MTDHIVKKAQ ECLPNIPLTV ISQGAEALVF YTDVHPYANE PYLQNRGKYV IKYRPSKPYR 70 80 90 100 110 120 HPKVDSSITK SRTVGEVKFM YKLNKLNINS PRIISADYNN GIIWMEYIGY SLPNGDVSSF 130 140 150 160 170 180 KNWLWYLERN NQDCTSESVE KMCFKVGQLI GKLHLHEMIH GDLTSSNILL NDDEPVLIDF 190 200 210 220 230 240 GLSSYSGLAE DRAVDLYVLE RAITSTHSVY AKEYNQWLLQ GYEDVHKHKE FGKQGQKKLV 250 260 EVLKKLDDVR LRGRKRSMLG

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382139 Genomic DNA. Translation: CAG90922.2.
RefSeq	XP_462413.2. XM_462413.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6BHA8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2905358.
GenomeReviews	Gene locus DEHA2G19998g in contig CR382139_GR.
KEGG	dha:DEHA2G19998g.
Phylogenomic databases
eggNOG	fuNOG08130.
HOGENOM	HBG574115.
OMA	MLLKPPL.
OrthoDB	EOG4R7ZKZ.
Family and domain databases
InterPro	IPR022495. Kinase-assoc_endopept-1. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
KO	K08851.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR03724. Arch_bud32. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS01245. RIO1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUD32_DEBHA

Accession

Primary (citable) accession number: Q6BHA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	December 16, 2008
Last modified:	November 16, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program