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Protein

Serine/threonine-protein phosphatase PP2A catalytic subunit 3

Gene

Ppn3

Organism
Paramecium tetraurelia
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Manganese 1By similarity
Metal bindingi64 – 641Manganese 1By similarity
Metal bindingi90 – 901Manganese 1By similarity
Metal bindingi90 – 901Manganese 2By similarity
Metal bindingi122 – 1221Manganese 2By similarity
Active sitei123 – 1231Proton donorBy similarity
Metal bindingi172 – 1721Manganese 2By similarity
Metal bindingi247 – 2471Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A catalytic subunit 3 (EC:3.1.3.16)
Short name:
PPN 3
Gene namesi
Name:Ppn3
ORF Names:GSPATT00000061001, PTMB.417c
OrganismiParamecium tetraurelia
Taxonomic identifieri5888 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
ProteomesiUP000000600 Componentsi: Chromosome, Partially assembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Serine/threonine-protein phosphatase PP2A catalytic subunit 3PRO_0000307836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei315 – 3151Leucine methyl esterBy similarity

Post-translational modificationi

Reversibly methyl esterified on Leu-315 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.

Keywords - PTMi

Methylation

Structurei

3D structure databases

ProteinModelPortaliQ6BFF6.
SMRiQ6BFF6. Positions 15-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000172696.
InParanoidiQ6BFF6.
KOiK04382.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6BFF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLNKLSSN DIGNIDRQIA KLKQGQILTE SEIKSLCIKA KEILSDEPNI
60 70 80 90 100
IQVRAPLTIC GDIHGQFHDL IELFQIGGNL PDTNYLFLGD YVDRGSQSVE
110 120 130 140 150
TFSLMLSLKV RYKDRIVLLR GNHENREINK VYGFYDECFR KYGNEIVWKQ
160 170 180 190 200
FTEVFGYLPL SAIVEQQIFC AHGGLSPAME SVDQIKQLNR VQDIPHEGLM
210 220 230 240 250
CDLLWSDPEE TKNGWGISPR GAGWTWGCDI TEKFLHSNKL KQIARAHQLV
260 270 280 290 300
MEGIQKVHNQ KTITIFSAPN YCYRCGNQAC IVEVDEQLRM NQTQFEPAPR
310
ENEPHTTRRV PDYFL
Length:315
Mass (Da):36,080
Last modified:August 16, 2004 - v1
Checksum:i19AD3EAF9F1E392D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR548612 Genomic DNA. Translation: CAH03615.1.
CT867985 Genomic DNA. Translation: CAK55626.1.
RefSeqiXP_001347241.1. XM_001347205.1.
XP_001423024.1. XM_001422987.1.
UniGeneiPte.28113.

Genome annotation databases

EnsemblProtistsiCAK55626; CAK55626; GSPATT00000061001.
GeneIDi2898390.
5008828.
KEGGiptm:GSPATT00000061001.
ptm:PTMB.417c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR548612 Genomic DNA. Translation: CAH03615.1.
CT867985 Genomic DNA. Translation: CAK55626.1.
RefSeqiXP_001347241.1. XM_001347205.1.
XP_001423024.1. XM_001422987.1.
UniGeneiPte.28113.

3D structure databases

ProteinModelPortaliQ6BFF6.
SMRiQ6BFF6. Positions 15-315.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiCAK55626; CAK55626; GSPATT00000061001.
GeneIDi2898390.
5008828.
KEGGiptm:GSPATT00000061001.
ptm:PTMB.417c.

Phylogenomic databases

HOGENOMiHOG000172696.
InParanoidiQ6BFF6.
KOiK04382.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Stock d4-2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Stock d4-2.

Entry informationi

Entry nameiPP2A3_PARTE
AccessioniPrimary (citable) accession number: Q6BFF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: August 16, 2004
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.